Anti-CD34抗体[QBEND-10] (ab8536)

概述

  • 产品名称Anti-CD34抗体[QBEND-10]
    参阅全部 CD34 一抗
  • 描述
    小鼠单克隆抗体[QBEND-10] to CD34
  • 特异性This antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immuno-gold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A.
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Human, Cynomolgus Monkey, Rhesus monkey
    不与反应: Rat, Sheep, Cow, Dog
  • 免疫原

    Other Immunogen Type corresponding to CD34. Human endothelial vesicles

  • 表位This antibody's target epitope is distinct from those of other anti-CD34 reactive mabs as demonstrated by its lack of sensitivity to neuraminidase treatment, and fluorescence staining profiles during flow cytometry analysis of target cell populations.
  • 阳性对照
    • tonsil, bone marrow

性能

应用

Our Abpromise guarantee covers the use of ab8536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50.

Mmethanol fixed cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.

靶标

  • 功能Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • 组织特异性Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • 序列相似性Belongs to the CD34 family.
  • 发展阶段On early hematopoietic progenitor cells.
  • 翻译后修饰Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Anti-CD34 antibody [QBEND-10] 图像

  • Anti-CD34 antibody [QBEND-10] (ab8536) at 5 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 100 kDa (possible glycosylated form).
  • ab8536 staining CD34 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in blocking buffer) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab8536 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8536, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in Jurkat cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.

    Detection system polymeric HRP. HIER with Pressure cooker pH6.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.

    Detection system polymeric HRP. HIER with Pressure cooker pH6.

  • ab8536 at 1/500 staining human umbilical cord tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with horse serum before incubation with the antibody for 16 hours. A biotinylated goat anti-mouse IgG was used as the secondary.

    See Abreview

Anti-CD34 antibody [QBEND-10] (ab8536)参考文献

This product has been referenced in:
  • Zhao W  et al. Effect of TGF-ß1 on the Migration and Recruitment of Mesenchymal Stem Cells after Vascular Balloon Injury: Involvement of Matrix Metalloproteinase-14. Sci Rep 6:21176 (2016). IHC-P ; Rat . Read more (PubMed: 26880204) »
  • El-Gendy R  et al. Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass® Scaffolds. Tissue Eng Part A 21:2034-43 (2015). IHC-P . Read more (PubMed: 25923923) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Kidney
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Sep 23 2016

Thank you for kindly confirming these details. I am sorry this vial of antibody has not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab8536 has been added to you...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hearthe customer has had difficulty obtaining satisfactory results from this vial of theantibody.

The details you have kindly provided will provide us ...

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Thank you for contacting us and sorry for the delay of our reply.

I have been informed that the datasheets have been corrected and updated.

ab49168,ab49515,ab49273, ab49759 : the unit sizes for these products are now inµL.
<...

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I have asked my colleagues in charge of the datasheets for the correct information regarding all these tissue culture supernatant antibodies.

I will contact you again as soon as I receive the right information. Sorry for the inconven...

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Thank you for contacting us.

Here is the additional information for flow cytometry - in addition to the figure legend given on the datasheet:

Methanol is used at 80% to fix the cells.The cells are blocked for 30minutes and there is...

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Thank you for your patience while I contacted the lab.

The Exact epiotpe site for ab8536 has not been mapped, so we are unsure if the epitope is intra- or extracellular. However we do know that the predominant staining is on the endothelial c...

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Thank you for contacting Abcam.

We have not tested ab8536 in flow cytometry in non-fixed cells, it may work in non-fixed cells but as we have not tested it, we cannot guarantee that it will work for you.

If you do test ab8536 in non...

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Thank you for contacting us. ab31630, can be used in IHC-P ab8536, can be used in IHC-P ab65865; IHC-P is yet not tested we are unable to confirm it. Can we suggest alternatives as ab34364, ab87951 and ab101500? ab33775; IHC-P is not a ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Umbilical cord)
Specification Umbilical cord
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization Yes - PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Manoj Kumar Valluru

Verified customer

提交于 Dec 04 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"