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Tissue, cells or virus corresponding to Mouse CD34. Specifically, the murine endothelioma cell line tEnd.
Our Abpromise guarantee covers the use of ab8158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
See Sho et al and Garlanda et al.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 0.5 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Staining with this method can be difficult, it has been reported to us that milder fixation methods for paraffin sections like using zinc solution work well (unfortunately we have no further detailed instructions of this fixation method).
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 41 kDa).|
|IHC-FrFl||Use at an assay dependent concentration. PubMed: 20382702|
|ELISA||Use at an assay dependent concentration.|
The main band is as expected at 80 kDa since the target is heavily glycosylated and phosphorylated.
ab8158 staining CD34 in mouse lung endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% BSA for 30 minutes at 4°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An undiluted Alexa Fluor® 555-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.
ab8158 staining CD34 in mouse mammary gland tissue sections by Immunohistochemistry (IHC-P - paraffin-embedded sections). Tissue was fixed with methacarnoy and blocked with 4% BSA for 60 minutes; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) for 16 hours. A undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.
ab8158 staining mouse brain tissue sections by IHC-P. Tissue sections were paraformaldehyde fixed and blocked with 0.5% Perkin-Elmer TNB Blocking Buffer for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated for 18 hours at 4°C. An biotin conjugated goat anti-rat was used as the secondary
Photomicrograph demonstrates CD34 in red and collagen type IV (ab19808) in blue in normal, adult brain vessels. Tissue was perfusion-fixed and cut into 15µm slide-mounted cryostat sections (i.e., lightly fixed, but not paraffin embedded).
Ab8158 at a dilution of 1/50 staining CD34 from mouse lung cells by Immunocytochemistry. The antibody was incubated with the sample for 1 hour and then detected using an Alexa-Fluor 488® goat anti-rat antibody. The staining for CD34 is shown in green. The samples were also stained for Smooth Muscle Actin (red stain).
This image is courtesy of an Abreview submitted on 8 February 2006.
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