Anti-CD34抗体[4H11(APG)], prediluted (Phycoerythrin) (ab18228)

概述

  • 产品名称Anti-CD34抗体[4H11(APG)], prediluted (Phycoerythrin)
    参阅全部 CD34 一抗
  • 描述
    小鼠单克隆抗体[4H11(APG)] to CD34, prediluted (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性The antibody reacts with Class III epitope on CD34 antibody. It completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia).

  • 阳性对照
    • CML cell line MOLM-7.

性能

应用

Our Abpromise guarantee covers the use of ab18228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 20µl for 106 cells. A cell suspension volume of 100 µl is recommended.



ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • 组织特异性Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • 序列相似性Belongs to the CD34 family.
  • 发展阶段On early hematopoietic progenitor cells.
  • 翻译后修饰Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Anti-CD34 antibody [4H11(APG)], prediluted (Phycoerythrin) 图像

  • ab18228 staining CD34 in KG-1a cell line by Flow Cytometery. Blue peak repersents negative control while red peak show signal with ab18228.

Anti-CD34 antibody [4H11(APG)], prediluted (Phycoerythrin) (ab18228)参考文献

This product has been referenced in:
  • Castro FV  et al. 5T4 oncofetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype. Leukemia 26:1487-98 (2012). Flow Cyt ; Human . Read more (PubMed: 22266911) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Fixation NONE
Sample Human Cell (B cell)
Specification B cell
Permeabilization No
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提交于 Feb 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"