概述

  • 产品名称Anti-CD33抗体[WM53]
    参阅全部 CD33 一抗
  • 描述
    小鼠单克隆抗体[WM53] to CD33
  • 经测试应用适用于: ICC, Flow Cyt, IP, WB, IHC-Frmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Human AML cells.

  • 阳性对照
    • Lymphatic tissue

性能

应用

Our Abpromise guarantee covers the use of ab30371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/50.
Flow Cyt 1/10 - 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. May also detects the smaller precursor protein at 40 kDa.
IHC-Fr 1/50 - 1/100.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
    • 组织特异性Monocytic/myeloid lineage cells.
    • 序列相似性Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 结构域Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • 翻译后修饰Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 33 antibody
      • CD33 antibody
      • CD33 antigen (gp67) antibody
      • CD33 antigen antibody
      • CD33 molecule antibody
      • CD33_HUMAN antibody
      • FLJ00391 antibody
      • gp67 antibody
      • My9 antibody
      • Myeloid cell surface antigen CD33 antibody
      • Myeloid cell surface antigen CD33 precursor antibody
      • Myeloid differentiation antigen CD33 antibody
      • p67 antibody
      • Sialic acid binding Ig like lectin 3 antibody
      • Sialic acid binding immunoglobulin like lectin 3 antibody
      • Sialic acid-binding Ig-like lectin 3 antibody
      • SIGLEC 3 antibody
      • Siglec-3 antibody
      • SIGLEC3 antibody
      see all

    Anti-CD33 antibody [WM53] 图像

    • ab30371 staining human peripheral blood monocytes by flow cytometry

    Anti-CD33 antibody [WM53] (ab30371)参考文献

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cell (Cytospin (human leukemia cells))
    Specification Cytospin (human leukemia cells)
    Fixative Acetone
    Permeabilization No
    Username

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    Verified customer

    提交于 May 22 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"