Anti-CD32B抗体(ab77093)

概述

  • 产品名称Anti-CD32B抗体
    参阅全部 CD32B 一抗
  • 描述
    山羊多克隆抗体to CD32B
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    PDALEEPDDQNRI

    by a Cysteine residue linker, corresponding to C terminal amino acids 298-310 of Human CD32 (FCGR2B)

  • 阳性对照
    • K562 cell lysate and U937 cell lysate. Lysate prepared from transfected HEK293 cells transiently expressing CD32B.
  • 常规说明


    This antibody is expected to recognise all four reported isoforms (NP_001002273; NP_001002274; NP_001002275; NP_003992).

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab77093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 3 - 6 µg/ml.

靶标

  • 功能Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
  • 组织特异性Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
  • 疾病相关Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
  • 序列相似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD32 antibody
    • CD32 antigen antibody
    • CD32B antibody
    • CDw32 antibody
    • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor antibody
    • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor for antibody
    • Fc gamma receptor IIB antibody
    • Fc gamma RII antibody
    • Fc gamma RIIB antibody
    • Fc-gamma RII antibody
    • Fc-gamma RII-b antibody
    • Fc-gamma-RIIb antibody
    • FCG2 antibody
    • FCG2B_HUMAN antibody
    • Fcgr2 antibody
    • FCGR2B antibody
    • FCRII antibody
    • FcRII-b antibody
    • IGFR2 antibody
    • IgG Fc receptor II beta antibody
    • IgG Fc receptor II-b antibody
    • Low affinity immunoglobulin gamma Fc region receptor II antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
    • Ly-17 antibody
    • Lym 1 antibody
    • Lymphocyte antigen 17 antibody
    see all

Anti-CD32B antibody 图像

  • Anti-CD32B antibody (ab77093) at 0.3 µg/ml + K562 lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ab77093 at 3.8 µg/ml staining CD32B in Human liver by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-CD32B antibody (ab77093)参考文献

ab77093 has not yet been referenced specifically in any publications.

Product Wall

We have three CD32B antibodies, I compared their immunogen sequences against the CD32A. Results are following: ab87965- Mouse monoclonal to CD32B homology with CD32A is 92%. Cross-reactivity with CD32A has not been tested. It is likely that there will...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"