概述

  • 产品名称
  • 描述
    兔多克隆抗体to CD320
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    fragment corresponding to C terminal amino acids 255-282 (AQERLRPLGLLVAMKESLLLSEQKTSLP) of Human CD320.

  • 阳性对照
    • Human lymph node tissue.

性能

应用

Our Abpromise guarantee covers the use of ab121433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin.
  • 组织特异性
    Expressed abundantly on follicular dendritic cells (FDCs).
  • 疾病相关
    Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC) [MIM:613646]; also called methylmalonic aciduria due to transcobalamin receptor defect. MMATC is a metabolic disorder characterized by increased blood C3-acylcarnitine levels, elevated methylmalonate and homocysteine, and low uptake of transcobalamin-bound cobalamin, but normal conversion to adenosylcobalamin and methylcobalamin. Plasma vitamin B12 and total homocysteine are normal.
  • 序列相似性
    Contains 2 LDL-receptor class A domains.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 8D6 antibody
    • 8D6 antigen antibody
    • 8D6A antibody
    • CD320 antibody
    • CD320 antigen antibody
    • CD320_HUMAN antibody
    • FDC-signaling molecule 8D6 antibody
    • FDC-SM-8D6 antibody
    • TCblR antibody
    • Transcobalamin receptor antibody
    see all

Anti-CD320 antibody 图像

  • ab121433 at a 1/50 dilution staining CD320 in paraffin-embedded Human lymph node tissue by Immunohistochemistry.

Anti-CD320 antibody (ab121433)参考文献

This product has been referenced in:
  • Fowler R  et al. Uptake and transport of B12-conjugated nanoparticles in airway epithelium. J Control Release 172:374-81 (2013). ICC/IF ; Human . Read more (PubMed: 24008152) »

See 1 Publication for this product

Product Wall

Abreviews
Application
Western blot
Sample
Human Purified protein (Hep G2 (Liver))
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (Stacking gel (10%), Resolving gel (10%))
Loading amount
20 µg
Treatment
10 concentrations of Vitamin B12 (500nM, 400nM, 200nM, 100nM, 50nM, 20nM, 10nM, 1nM, 100pM and 25pM) for 10 days
Specification
Hep G2 (Liver)
Blocking step
(agent) for 12 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 4°C
Username

Joseph Boachie

Verified customer

提交于 Aug 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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