概述

  • 产品名称Anti-CD32抗体[EPR6658]
    参阅全部 CD32 一抗
  • 描述
    兔单克隆抗体[EPR6658] to CD32
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    A synthetic peptide, corresponding to residues in Human CD32.

  • 阳性对照
    • K562 and Human platelet lysates; Human lung and spleen tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab125013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 37-43 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
    • 组织特异性Found on monocytes, neutrophils and eosinophil platelets.
    • 序列相似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD32 antibody
      • CD32A antibody
      • CD32B antibody
      • CD32C antibody
      • CDw32 antibody
      • Fc fragment of IgG low affinity IIa receptor antibody
      • Fc fragment of IgG low affinity IIa receptor for (CD32) antibody
      • Fc fragment of IgG low affinity IIb receptor antibody
      • Fc fragment of IgG low affinity IIb receptor for (CD32) antibody
      • Fc fragment of IgG low affinity IIc receptor antibody
      • Fc fragment of IgG low affinity IIc receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
      • Fc gamma receptor IIB antibody
      • Fc gamma receptor IIC antibody
      • Fc gamma RII a antibody
      • Fc gamma RII b antibody
      • Fc gamma RII c antibody
      • Fc gamma RIIb antibody
      • Fc gamma RIIc antibody
      • Fc receptor, IgG, low affinity IIb antibody
      • Fc-gamma RII-a antibody
      • Fc-gamma RII-b antibody
      • Fc-gamma RII-c antibody
      • Fc-gamma-RIIa antibody
      • FC-gamma-RIIB antibody
      • Fc-GAMMA-RIIC antibody
      • Fc[g]RII antibody
      • FCG2 antibody
      • FCG2A_HUMAN antibody
      • FcGR antibody
      • FCGR2 antibody
      • FCGR2A antibody
      • FCGR2A1 antibody
      • FCGR2B antibody
      • FCGR2C antibody
      • Fcgr3 antibody
      • Fcgr3a antibody
      • FcgRII antibody
      • Fcr-2 antibody
      • Fcr-3 antibody
      • FcRII a antibody
      • FCRII antibody
      • FcRII b antibody
      • FcRII c antibody
      • FcRII-a antibody
      • FcRIIC antibody
      • IGFR2 antibody
      • IgG Fc receptor II a antibody
      • IgG Fc receptor II b antibody
      • IgG Fc receptor II beta antibody
      • IgG Fc receptor II c antibody
      • IgG Fc receptor II-a antibody
      • IgG Fc receptor II-b antibody
      • IgG Fc receptor II-c antibody
      • Immunoglobulin G Fc receptor II antibody
      • Immunoglobulin G Fc receptor II c antibody
      • Low affinity immunoglobulin gamma Fc region receptor II antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-c antibody
      • Ly-17 antibody
      • Ly-m20 antibody
      • LyM-1 antibody
      • Lymphocyte antigen 17 antibody
      see all

    Anti-CD32 antibody [EPR6658] 图像

    • All lanes : Anti-CD32 antibody [EPR6658] (ab125013) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Human platelet lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
      Observed band size : 37-43 kDa (why is the actual band size different from the predicted?)
    • ab125013 at 1/250 staining CD32 in paraffin-embedded Human lung tissue by immunohistochemistry.
    • ab125013 at 1/250 staining CD32 in paraffin-embedded Human spleen tissue by immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-CD32 antibody [EPR6658] (ab125013)参考文献

    This product has been referenced in:
    • Murinello S  et al. Fc? receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration. Invest Ophthalmol Vis Sci 55:247-58 (2014). IHC-P ; Human . Read more (PubMed: 24334446) »

    See 1 Publication for this product

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