概述

  • 产品名称Anti-CD32抗体[AT10]
    参阅全部 CD32 一抗
  • 描述
    小鼠单克隆抗体[AT10] to CD32
  • 经测试应用适用于: Neutralising, Flow Cyt, IHC-Fr, IP, Blockingmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog
  • 免疫原

    K562 cell line

性能

应用

Our Abpromise guarantee covers the use of ab41899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Neutralising Use at an assay dependent concentration. PubMed: 20713893
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use a concentration of 20 µg/ml.
IP Use a concentration of 20 µg/ml.
Blocking Use at an assay dependent concentration.

靶标

  • 功能Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
  • 组织特异性Found on monocytes, neutrophils and eosinophil platelets.
  • 序列相似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD32 antibody
    • CD32A antibody
    • CD32B antibody
    • CD32C antibody
    • CDw32 antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIa receptor for (CD32) antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIb receptor for (CD32) antibody
    • Fc fragment of IgG low affinity IIc receptor antibody
    • Fc fragment of IgG low affinity IIc receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
    • Fc gamma receptor IIB antibody
    • Fc gamma receptor IIC antibody
    • Fc gamma RII a antibody
    • Fc gamma RII b antibody
    • Fc gamma RII c antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIIc antibody
    • Fc receptor, IgG, low affinity IIb antibody
    • Fc-gamma RII-a antibody
    • Fc-gamma RII-b antibody
    • Fc-gamma RII-c antibody
    • Fc-gamma-RIIa antibody
    • FC-gamma-RIIB antibody
    • Fc-GAMMA-RIIC antibody
    • Fc[g]RII antibody
    • FCG2 antibody
    • FCG2A_HUMAN antibody
    • FcGR antibody
    • FCGR2 antibody
    • FCGR2A antibody
    • FCGR2A1 antibody
    • FCGR2B antibody
    • FCGR2C antibody
    • Fcgr3 antibody
    • Fcgr3a antibody
    • FcgRII antibody
    • Fcr-2 antibody
    • Fcr-3 antibody
    • FcRII a antibody
    • FCRII antibody
    • FcRII b antibody
    • FcRII c antibody
    • FcRII-a antibody
    • FcRIIC antibody
    • IGFR2 antibody
    • IgG Fc receptor II a antibody
    • IgG Fc receptor II b antibody
    • IgG Fc receptor II beta antibody
    • IgG Fc receptor II c antibody
    • IgG Fc receptor II-a antibody
    • IgG Fc receptor II-b antibody
    • IgG Fc receptor II-c antibody
    • Immunoglobulin G Fc receptor II antibody
    • Immunoglobulin G Fc receptor II c antibody
    • Low affinity immunoglobulin gamma Fc region receptor II antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-c antibody
    • Ly-17 antibody
    • Ly-m20 antibody
    • LyM-1 antibody
    • Lymphocyte antigen 17 antibody
    see all

Anti-CD32 antibody [AT10] 图像

  • Overlay histogram showing K562 cells stained with ab41899 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41899, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed K562 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD32 antibody [AT10] (ab41899)参考文献

This product has been referenced in:
  • Karagiannis P  et al. IgG4 subclass antibodies impair antitumor immunity in melanoma. J Clin Invest 123:1457-74 (2013). IHC-Fr ; Human . Read more (PubMed: 23454746) »
  • Municio C  et al. The response of human macrophages to ß-glucans depends on the inflammatory milieu. PLoS One 8:e62016 (2013). IP ; Human . Read more (PubMed: 23637950) »

See all 11 Publications for this product

Product Wall

Thank you for contacting us.

The IP protcol could be found at the following link;

http://www.abcam.com/index.html?pageconfig=resource&rid=11385

I hope this information is helpful to you. Please do not hesitate to contact ...

Read More

Thank you very much for your interest in ab41899 Anti-CD32 antibody [AT10] - Azide free.

According to Bunk S et al., this antibody completly blocksFcγRII (CD32) in human PBMCs.Please see the attachment.

To our knowledge, ab418...

Read More

Thank you very much for your interest in our products. ab89191 (Anti-CD32 antibody [MM0270- 3L1]) from our catalogue was raised using CD32b extra cellular domain and reacts with both CD32a and CD32b. Unfortunately, it was not specifically tested ...

Read More

Thank you for your enquiry. I can confirm the following details which I have obtained from the laboratories: ab41899 CD32 antibody: Unfortunately, we do not have a specific protocol for the use of this product in blocking experiments, this appl...

Read More

As far as I am aware, there has not been a study formally identifying the minimal epitoperecognised by this antibody. However, there is some information on the epitope recognised in the original paper describing the antibody: Greenman J, Tutt AL, G...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"