概述

  • 产品名称Anti-CD31 (phospho Y713)抗体
    参阅全部 CD31 一抗
  • 描述
    兔多克隆抗体to CD31 (phospho Y713)
  • 特异性Detects endogenous levels of CD31 only when phosphorylated at tyrosine 713 (Human: Tyr713; Mouse: Tyr702).
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human CD31 around the phosphorylation site of tyrosine 713 (T-V-YP-S-E).

  • 阳性对照
    • HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab62169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/500.
ELISA 1/40000.
WB 1/500 - 1/1000. Predicted molecular weight: 82 kDa.

靶标

  • 功能Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 组织特异性Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 序列相似性Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻译后修饰Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 细胞定位Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

Anti-CD31 (phospho Y713) antibody 图像

  • All lanes : Anti-CD31 (phospho Y713) antibody (ab62169) at 1/500 dilution

    Lane 1 : HepG2 cells
    Lane 2 : HepG2 cells with blocked with the phospho peptide


    Predicted band size : 82 kDa
  • Immunofluorescence analysis of HeLa cells, using CD31 (phospho Y713) antibody (ab62169) at 1/100 - 1/500 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.

Anti-CD31 (phospho Y713) antibody (ab62169)参考文献

This product has been referenced in:
  • Bayes-Genis A  et al. Human progenitor cells derived from cardiac adipose tissue ameliorate myocardial infarction in rodents. J Mol Cell Cardiol 49:771-80 (2010). ICC/IF ; Human . Read more (PubMed: 20713059) »

See 1 Publication for this product

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