概述

  • 产品名称Anti-CD31抗体[P2B1]
    参阅全部 CD31 一抗
  • 描述
    小鼠单克隆抗体[P2B1] to CD31
  • 经测试应用适用于: ICC/IF, IHC - Wholemount, WB, IPmore details
    不适用于: IHC-Fr or IHC-P
  • 种属反应性
    与反应: Mouse, Human
    不与反应: Cow
  • 免疫原

    Tissue/ cell preparation (Human) - IL-1beta activated human umbilical vein endothelial cells (HUVECs).

  • 常规说明

    ab24590 activates the binding of human T-lymphocytes to endothelial cells.

性能

应用

Our Abpromise guarantee covers the use of ab24590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/1000.
IHC - Wholemount 1/100.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 83 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

IP 1/1000.
  • 应用说明Is unsuitable for IHC-Fr or IHC-P.
  • 靶标

    • 功能Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
    • 组织特异性Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
    • 序列相似性Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • 结构域The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
    • 翻译后修饰Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
    • 细胞定位Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Adhesion molecule antibody
      • CD31 antibody
      • CD31 antigen antibody
      • CD31 EndoCAM antibody
      • EndoCAM antibody
      • FLJ34100 antibody
      • FLJ58394 antibody
      • GPIIA antibody
      • GPIIA' antibody
      • PECA1 antibody
      • PECA1_HUMAN antibody
      • Pecam 1 antibody
      • PECAM 1 CD31 EndoCAM antibody
      • PECAM antibody
      • PECAM-1 antibody
      • Pecam1 antibody
      • Platelet and endothelial cell adhesion molecule 1 antibody
      • Platelet endothelial cell adhesion molecule antibody
      • Platelet/endothelial cell adhesion molecule 1 antibody
      see all

    Anti-CD31 antibody [P2B1] 图像

    • Positive immunocytochemical staining of human primary choroidal endothelial cells using ab24590 at a dilution of 1/100. The tissue was fixed with paraformaldehyde and the antibody was incubated for 3 hours. An Alexa Fluor® 488 goat anti-mouse antibody was used as a secondary antibody.

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    • Images of isolated capillaries triple labeled for EC marker protein CD31 (red channel), PC marker protein NG-2 (green channel), and Hoechst for nuclei (blue channel).

      Cochlear stria vascularis capillaries were isolated from 6-week-old CBA/CaJ mice. The capillary isolation was achieved with a special procedure we developed and named the “sandwich-dissociation” method. The tissue samples were fixed in 4% paraformaldehyde at 4°C for 2 h, washed in PBS for 30 min, permeabilized in 0.5% Triton X-100, then immunoblocked in a solution of 10% goat serum in 1% bovine albumin in PBS for 30 min. The specimens were incubated with primary antibodies and secondary antibodies (goat Alexa Fluo 488-conjugated anti-mouse and anti-rabbit IgG antibodies).

    • ab24590 staining CD31 in the HEK 293T cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton and blocked with 3% BSA for 1 hour at RT. Samples were incubated with primary antibody (1/50 in PBS + 3% BSA) for 1 hour. A Alexa Fluor® 555-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody (1/500).

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    • All lanes : Anti-CD31 antibody [P2B1] (ab24590) at 5 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) WCL
      Lane 2 : THP1 (Human acute monocytic leukemia cell line) WCL
      Lane 3 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
      Lane 4 : Human Platelets

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size : 83 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 4 minutes

      We recommend using 3% milk as the blocking agent for Western blot.

    Anti-CD31 antibody [P2B1] (ab24590)参考文献

    This product has been referenced in:
    • Eckert A  et al. Bystander Effect Fuels Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells to Quickly Attenuate Early Stage Neurological Deficits After Stroke. Stem Cells Transl Med 4:841-51 (2015). IHC-FrFl ; Mouse . Read more (PubMed: 26025980) »
    • Zhao X  et al. Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction. Am J Physiol Heart Circ Physiol 309:H702-10 (2015). IHC-P ; Mouse . Read more (PubMed: 26071543) »

    See all 23 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Pig Cell lysate - whole cell (PAE cells)
    Specification PAE cells
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    提交于 Jan 21 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Fish Tissue sections (Heart)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate pH6.0
    Permeabilization Yes - Triton X
    Specification Heart
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
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    提交于 Dec 24 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Human fibromatosis)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Permeabilization Yes - Tween-20
    Specification Human fibromatosis
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative Paraformaldehyde
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    提交于 Dec 15 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (mouse liver)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Permeabilization Yes - Tween-20
    Specification mouse liver
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative Formaldehyde
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    提交于 Dec 08 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample Human Cell (HEK 293T)
    Specification HEK 293T
    Permeabilization Yes - Triton 1%
    Fixative Formaldehyde
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    提交于 Nov 18 2014

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Peripheral blood lymphocyte (PBL))
    Specification Peripheral blood lymphocyte (PBL)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative Acetone
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    提交于 Oct 22 2014

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing
    Sample Rat Tissue lysate - whole (liver)
    Specification liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    提交于 Oct 22 2014

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Tissue lysate - whole (liver)
    Specification liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    提交于 Oct 22 2014

    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Liver)
    Specification Liver
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative Acetone
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    提交于 Oct 20 2014

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample Mouse Tissue sections (Liver)
    Specification Liver
    Permeabilization No
    Fixative Acetone
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    提交于 Oct 20 2014

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