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Mouse t.end.1 cells (polyoma middle T (PmT)-transformed EC)
Our Abpromise guarantee covers the use of ab7388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
No fixation or permeabilization needed. Epitope is sensitive to trypsin!
|ICC/IF||Use at an assay dependent concentration. Please see reviews for fixative recommendations. Certain users find acetone fixation necessary while others find 4% PFA necessary. In addition, found to work at 1/1000 dilution.|
|IP||Use at an assay dependent concentration.|
Immunohistochemical analysis of frozen mouse skin tissue, staining CD31 with ab7388.
Tissue was fixed with acetone and blocked with 2% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300 in 1% BSA) for 4 hours at 20°C. An Alexa Fluor® 488-conjugated goat anti-rat monoclonal IgG (1/500) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"