概述

  • 产品名称Anti-CD31抗体[JC/70A]
    参阅全部 CD31 一抗
  • 描述
    小鼠单克隆抗体[JC/70A] to CD31
  • 经测试应用适用于: IHC-Fr, ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human, Cynomolgus Monkey
  • 免疫原

    Membrane preparation of a spleen from a patient with hairy cell leukaemia.

  • 阳性对照
    • Tonsil.

应用

Our Abpromise guarantee covers the use of ab9498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20618694
Flow Cyt 1/20.

Paraformaldehyde or methanol fixed cells (also see PMID: 20618694).

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 82 kDa).
IHC-P 1/10 - 1/25. 30-60mins at RT (ABC method). Biopsy specimens should be fixed in periodate-lysine-paraformaldehyde solution or 10% formalin solution. Perform enzymatic antigen retrieval (pepsin or 0.1% trypsin solution at 37°C for 60 min (see reference by Moriyama M et al) before commencing with IHC staining protocol.

靶标

  • 功能Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 组织特异性Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 序列相似性Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻译后修饰Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 细胞定位Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

Anti-CD31 antibody [JC/70A] 图像

  • ab9498 staining human tonsil by IHC-P.

  • ab9498 staining CD31 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.
  • Overlay histogram showing Jurkat cells stained with ab9498 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9498, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • ab9498 at 1/100 staining human lymph node tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat based antigen retrieval step was performed. The tissue was then blocked with serum and incubated with ab9498 overnight. An HRP conjugated goat antibody was used as the secondary.

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  • Formalin fixed paraffin embedded human tonsil stained with CD31, using ABC and AEC chromagen.

Anti-CD31 antibody [JC/70A] (ab9498)参考文献

This product has been referenced in:
  • Schuhmann MK  et al. CD28 superagonist-mediated boost of regulatory T cells increases thrombo-inflammation and ischemic neurodegeneration during the acute phase of experimental stroke. J Cereb Blood Flow Metab 35:6-10 (2015). IHC ; Mouse . Read more (PubMed: 25315859) »
  • Raha-Chowdhury R  et al. Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci 16:24 (2015). IHC . Read more (PubMed: 25896789) »

See all 19 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Ovary)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium citrate 10 mM, pH 6.0
Permeabilization Yes - 0.025% Triton X-100
Specification Ovary
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
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提交于 Sep 27 2016

Application Immunocytochemistry
Sample Human Cultured Cells (Primary Human Internal Thoracic Artery Endothelia)
Permeabilization Yes - Cold Methanol
Specification Primary Human Internal Thoracic Artery Endothelia
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Sep 25 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Antigen retrieval solution pH 9 of DAKO
Permeabilization No
Specification Lung
Blocking step Fetal calf serum (filtered) as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde
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Dr. Thibault Geinoz

Verified customer

提交于 Jun 27 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (Endothelial cells (PAEC))
Specification Endothelial cells (PAEC)
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Thibault Geinoz

Verified customer

提交于 Nov 21 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step None
Sample Rabbit Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde
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提交于 Jun 04 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Tissue lysate - whole (Skeletal Muscle)
Specification Skeletal Muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Feb 28 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Pepsin
Sample Rabbit Tissue sections (Adipose)
Specification Adipose
Permeabilization No
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Jan 27 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Cit (ph6)
Sample Human Tissue sections (Skeletal Muscle)
Specification Skeletal Muscle
Permeabilization No
Fixative Methacarn
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Verified customer

提交于 Jan 27 2014



We have not previously tested this antibody on rat samples and therefore we cannot guarantee that this will work.

I have compared the sequence homology between the human and rat protein and there in only 65% sequence homology. We usu...

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Unfortunately, ab9498 is an unpurified antibody. We can only provide concentrations for purified antibodies as unpurified antibody preparations vary significantly in specific antibody concentration.

Nevertheless, I recommend you having a look...

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1-10 of 24 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"