概述

  • 产品名称Anti-CD31抗体
    参阅全部 CD31 一抗
  • 描述
    兔多克隆抗体to CD31
  • 经测试应用适用于: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Mouse CD31 (C terminal).

  • 阳性对照
    • Jurkat and Raji cells, Tonsil.

应用

Our Abpromise guarantee covers the use of ab28364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/50.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/20. PubMed: 17316380
IHC-FoFr Use at an assay dependent concentration.
WB 1/500.

See Abreview. Customer feedback indicates that there might be some batch-to-batch variation with respect to mouse cross-reactivity. Please contact Abcam if you have any queries.

靶标

  • 功能Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 组织特异性Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 序列相似性Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻译后修饰Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 细胞定位Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

Anti-CD31 antibody 图像

  • ab28364 at a 1/300 dilution, staining human CD31 in fetal heart tissue by Immunohistochemistry, frozen tissue.

    See Abreview

  • ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • Anti-CD31 antibody (ab28364) at 1/500 dilution + Human vascular endothelial cells at 25 µg

    Secondary
    anti-rabbit HRP at 1/5000 dilution

    This image is courtesy of an anonymous Abreview

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  • Immunofluorescent analysis of 3% formaldehyde-fixed,NP40 permeabilized Saos cells labeling CD31 with ab28364 at 1/25 dilution,followed by secondary antibody at 1/500 dilution.

  • ab28364 staining CD31 in mouse xenograft tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices and blocked with 1% BSA for 60 minutes at 21°C. Antigen retrieval was by heat mediation in a Tris/EDTA pH9 buffer. The sample was incubated with primary antibody (20 ug/mL) at 4°C for 16 hours. A HRP-conjugated goat anti-rabbit polyclonal (2 ug/mL) was used as the secondary antibody.

  • Anti-CD31 antibody (ab28364) at 1/50 dilution staining mouse tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was formaldehyde fixed and blocked with serum. The tissue was incubated with the primary antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary. The endothelium of vessels of any tissues are positive.

    See Abreview

Anti-CD31 antibody (ab28364)参考文献

This product has been referenced in:
  • Doi H  et al. Potency of umbilical cord blood- and Wharton's jelly-derived mesenchymal stem cells for scarless wound healing. Sci Rep 6:18844 (2016). IHC ; Mouse . Read more (PubMed: 26728342) »
  • Herath NI  et al. The DNA Repair Inhibitor DT01 as a Novel Therapeutic Strategy for Chemosensitization of Colorectal Liver Metastasis. Mol Cancer Ther 15:15-22 (2016). Read more (PubMed: 26637369) »

See all 380 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Aug 26 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (stomach)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization No
Specification stomach
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative 10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Aug 23 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (stomach)
Permeabilization No
Specification stomach
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Aug 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Aug 09 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (artery)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization No
Specification artery
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Aug 09 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (artery)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization No
Specification artery
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Aug 09 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH 8.0
Permeabilization No
Specification Breast tumor
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Aug 09 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (Ovale follicle wall)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization No
Specification Ovale follicle wall
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization No
Specification spleen
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 21°C
Fixative 10% NBF
Username

Abcam user community

Verified customer

提交于 Jul 28 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dasypus novemcinctus Tissue sections (Uterus (non-gravid))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate buffer
Permeabilization No
Specification Uterus (non-gravid)
Blocking step BSA as blocking agent for 5 minute(s) · Concentration: 0.1% · Temperature: 25°C
Fixative Formaldehyde
Username

Arun Chavan

Verified customer

提交于 Apr 18 2016

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"