Anti-CD31抗体[390] (Phycoerythrin) (ab25644)

概述

  • 产品名称Anti-CD31抗体[390] (Phycoerythrin)
    参阅全部 CD31 一抗
  • 描述
    大鼠单克隆抗体[390] to CD31 (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性ab25644 recognises Mouse CD31/PECAM-1.
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Mouse leukocyte cell line 32D

  • 常规说明


    ab25644 inhibits the aggregation of L cells transfected with a variant form of CD31.

应用

Our Abpromise guarantee covers the use of ab25644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.2µg for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

  • 功能Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 组织特异性Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 序列相似性Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻译后修饰Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 细胞定位Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

Anti-CD31 antibody [390] (Phycoerythrin) 图像

  • ab25644 staining CD31 in Mouse stem cell derived endothelial cells by Flow Cytometry. Cells were detached, washed, centrifuged and resuspended in 10% FCS/PBS/0.1 mM EDTA and incubated for 30 minutes on ice.  The sample was incubated with the primary antibody (0.5 µg per 1 x 106 cells) for 30 minutes at 4°C in the dark.  An Isotype-matched IgG-PE antibody was used as control. After adding 1.5ml cold 5% FCS/PBS/0.1 mM EDTA  each tube was mixed by inversion and centrifuged; the supernatant was aspirated.  After repeat washing the cells were resuspended in 400µl cold 1% FCS/PBS/0.1 mM EDTA and analyzed.

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Anti-CD31 antibody [390] (Phycoerythrin) (ab25644)参考文献

This product has been referenced in:
  • Young JA  et al. Regulation of vascular leak and recovery from ischemic injury by general and VE-cadherin-restricted miRNA antagonists of miR-27. Blood 122:2911-9 (2013). Read more (PubMed: 24009229) »

See 1 Publication for this product

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To our knowledge,the following antibodies you listed have not been tested in IHC-P. Tested applications and species are listed on the individual datasheets. These are updated as soon as we receive any new information. You may wish to use our AbTrial pr...

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I would recommend our rat IgG2a isotype control that is PE conjugated, an134674 http://www.abcam.com/rat-igg2a-kappa-monoclonal-klhg2a-1-1-phycoerythrin-isotype-control-ab134674.html

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Stem cell derived endothelial cell)
Specification Stem cell derived endothelial cell
Permeabilization No
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Dr. John Paul Kirton

Verified customer

提交于 Oct 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"