antigen sequence: DDADSYWCGT ERPGIDLGVK VQVTINPGTQ TAVSEWTTTT ASLAFTAAAT QKTSSPLTRS PLKSTH, corresponding to amino acids 100-165 of Human CD300LD (UniProt ID: Q6UXZ3).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CD300LD antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-CD300LD antibody (ab121955)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (microtubules). Recommended concentration of ab121955 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD300LD antibody (ab121955)
ab121955, at a 1/10 dilution, staining CD300LD in paraffin emebedded Human placenta tissue by Immunohistochemistry.
Anti-CD300LD antibody (ab121955)参考文献
has not yet been referenced specifically in any publications.
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