internal sequence amino acids 115-183 (PWLRDFHDPI VEVEVSVFPA GTTTASSPQS SMGTSGPPTK LPVHTWPSVT RKDSPEPSPH PGSLFSNVR) of Human CD300C.
- Human spleen tissue; Transfected HEK293T cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Present on the surface of monocytes, neutrophils, a proportion of peripheral blood T- and B-lymphocytes and lymphocytic cell lines.
Belongs to the CD300 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Information by UniProt
- CD300 antigen-like family member C antibody
- CD300c antibody
- CD300c antigen antibody
Immunocytochemistry/ Immunofluorescence - Anti-CD300C antibody (ab121435)
Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei. Recommended concentration of ab121435 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab121435)
ab121435 at a 1/250 dilution staining CD300C in paraffin-embedded Human spleen tissue by Immunohistochemistry.
Western blot - Anti-CD300C antibody (ab121435)
All lanes : Anti-CD300C antibody (ab121435) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : CD300C transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa)
Developed using the ECL technique
has not yet been referenced specifically in any publications.
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