Anti-CD300抗体[MEM-260] (ab53317)

概述

  • 产品名称Anti-CD300抗体[MEM-260]
    参阅全部 CD300 一抗
  • 描述
    小鼠单克隆抗体[MEM-260] to CD300
  • 特异性ab53317 recognizes CD300a, (Customer feedback suggests it also cross-reacts with CD300c, however we have not yet tested it for CD300c). The antibody MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    HPB human lymphoid leukemia cell line.

  • 阳性对照
    • Positive material: HPB cell line. Negative material: Jurkat human cell line, Raji human cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from hybridoma culture supernatant by protein-A affinity chromatography.
  • 克隆单克隆
  • 克隆编号MEM-260
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 35 kDa. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Flow Cyt Use a concentration of 2 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Acts as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation.
  • 组织特异性Highly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocytes and monocyte-related cells.
  • 序列相似性Belongs to the CD300 family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻译后修饰Phosphorylated on tyrosine.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD300 antigen like family member antibody
    • CD300 antigen-like family member F antibody
    • CD300 molecule like family member f antibody
    • CD300f antibody
    • CD300LF antibody
    • CLM antibody
    • CLM-1 antibody
    • CLM1 antibody
    • CLM1_HUMAN antibody
    • CMRF35-like molecule 1 antibody
    • IGSF antibody
    • IgSF13 antibody
    • Immune receptor expressed on myeloid cells 1 antibody
    • immune receptor expressed on myeloid cells antibody
    • immunoglobin superfamily member antibody
    • Immunoglobulin superfamily member 13 antibody
    • Immunoglobulin superfamily member antibody
    • Inhibitory receptor IREM1 antibody
    • IREM antibody
    • IREM-1 antibody
    • IREM1 antibody
    • Nepmucin antibody
    • NK inhibitory receptor antibody
    • NKIR antibody
    • TREM antibody
    • triggering receptors expressed on myeloid cells antibody
    • UNQ3105/PRO10111 antibody
    see all

Anti-CD300 antibody [MEM-260] (ab53317)参考文献

ab53317 has not yet been referenced specifically in any publications.

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