Anti-CD30抗体[CON6D/ C2] (ab76518)

概述

  • 产品名称Anti-CD30抗体[CON6D/ C2]
    参阅全部 CD30 一抗
  • 描述
    小鼠单克隆抗体[CON6D/ C2] to CD30
  • 经测试应用适用于: IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Hodgkin's lymphoma cell line L428

  • 阳性对照
    • Hodgkin’s lymphoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab76518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50. 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
  • 序列相似性Contains 6 TNFR-Cys repeats.
  • 翻译后修饰Phosphorylated on serine and tyrosine residues.
  • 细胞定位Cytoplasm and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 30 antibody
    • CD30 antibody
    • CD30 antigen antibody
    • CD30L receptor antibody
    • Cytokine receptor CD30 antibody
    • D1S166E antibody
    • KI 1 antibody
    • KI 1 antigen antibody
    • Ki-1 antigen antibody
    • KI1 antibody
    • Lymphocyte activation antigen CD30 antibody
    • TNFRSF 8 antibody
    • Tnfrsf8 antibody
    • TNR8_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 8 antibody
    see all

Anti-CD30 antibody [CON6D/ C2] 图像

  • Overlay histogram showing MOLT4 cells stained with ab76518 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76518, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MOLT4 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-CD30 antibody [CON6D/ C2] (ab76518)参考文献

ab76518 has not yet been referenced specifically in any publications.

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Regarding the purity of ab76518, I verified with the lab that the product’s constitution does NOT contain gelatin or carrier proteins. Therefore, this BSA and Azide free anti-CD30 antibody should be suitable...

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Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Hu...

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