Anti-CD30抗体[Ber-H2], prediluted (ab871)

概述

  • 产品名称Anti-CD30抗体[Ber-H2], prediluted
    参阅全部 CD30 一抗
  • 描述
    小鼠单克隆抗体[Ber-H2] to CD30, prediluted
  • 特异性This antibody reacts with mononuclear cells in Hodgkin lymphoma, Reed-Sternberg cells and most anaplastic large cell lymphomas. It stains large cells localized around the B cell in the lymphoid tissues. This antibody stains the cell surface and the golgi region in the cells.
  • 经测试应用适用于: IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    BALB/C mice were injected with L428 cell line cells.

性能

应用

Our Abpromise guarantee covers the use of ab871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
IHC-Fr
  • 应用说明This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
    Enzymatic predigestion with proteolytic enzymes may increase staining intensity.
    Although not required, microwave treatment will considerably enhance the signal.
  • 靶标

    • 功能Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
    • 序列相似性Contains 6 TNFR-Cys repeats.
    • 翻译后修饰Phosphorylated on serine and tyrosine residues.
    • 细胞定位Cytoplasm and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 30 antibody
      • CD30 antibody
      • CD30 antigen antibody
      • CD30L receptor antibody
      • Cytokine receptor CD30 antibody
      • D1S166E antibody
      • KI 1 antibody
      • KI 1 antigen antibody
      • Ki-1 antigen antibody
      • KI1 antibody
      • Lymphocyte activation antigen CD30 antibody
      • TNFRSF 8 antibody
      • Tnfrsf8 antibody
      • TNR8_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 8 antibody
      see all

    Anti-CD30 antibody [Ber-H2], prediluted 图像

    • IHC image of ab871 staining in Hodgkin lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-CD30 antibody [Ber-H2], prediluted (ab871)参考文献

    ab871 has not yet been referenced specifically in any publications.

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