概述

  • 产品名称
  • 描述
    鸡多克隆抗体to CD30
  • 宿主
    Chicken
  • 特异性
    ab26864 recognises CD30 protein.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Fusion protein: SGTIPQAKPT PVSPATSSAS TMPVRGGTRL AQEAASKLTR APDSPSSVGR PSSDPG, corresponding to amino acids 171-226 of Human CD30

性能

应用

Our Abpromise guarantee covers the use of ab26864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 64 kDa).

靶标

  • 功能
    Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
  • 序列相似性
    Contains 6 TNFR-Cys repeats.
  • 翻译后修饰
    Phosphorylated on serine and tyrosine residues.
  • 细胞定位
    Cytoplasm and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 30 antibody
    • CD30 antibody
    • CD30 antigen antibody
    • CD30L receptor antibody
    • Cytokine receptor CD30 antibody
    • D1S166E antibody
    • KI 1 antibody
    • KI 1 antigen antibody
    • Ki-1 antigen antibody
    • KI1 antibody
    • Lymphocyte activation antigen CD30 antibody
    • TNFRSF 8 antibody
    • Tnfrsf8 antibody
    • TNR8_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 8 antibody
    see all

图片

  • Anti-CD30 antibody (ab26864) at 1/2000 dilution + human CD30 fusion protein (amino acids 171-226 )

    Secondary
    HRP conjugated goat anti IgY/Fc at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 64 kDa
    Observed band size: 46 kDa (why is the actual band size different from the predicted?)



    Method:
    Block membrane with 5% non-fat milk in PBS-T for 1 hour at room temperature or longer at 4°C
    Incubate membrane with ab26864 at 1/2000 dilution with 1% milk in PBS-T at RT for 1 hour
    Rinse 3 times with PBS-T, then wash membrane 3 times for 5 mins with PBS-T
    Incubate with HRP-conjugated goat anti IgY/Fc at 1/1000 dilution for with 1% milk PBS-T at RT for 1 hour
    Rinse 3 times with PBS-T, then wash 3 times for 5 mins with shaking, before ECL detection

实验方案

文献

ab26864 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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