ab26864 recognises CD30 protein.
Fusion protein: SGTIPQAKPT PVSPATSSAS TMPVRGGTRL AQEAASKLTR APDSPSSVGR PSSDPG, corresponding to amino acids 171-226 of Human CD30
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Concentration information loading...
Protein L purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 64 kDa).
Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
Contains 6 TNFR-Cys repeats.
Phosphorylated on serine and tyrosine residues.
Cytoplasm and Cell membrane.
Information by UniProt
CD 30 antibody
CD30 antigen antibody
Western blot - CD30 antibody (ab26864)
Anti-CD30 antibody (ab26864) at 1/2000 dilution + human CD30 fusion protein (amino acids 171-226 )
HRP conjugated goat anti IgY/Fc at 1/1000 dilution
Developed using the ECL technique.
Predicted band size:
Observed band size:
46 kDa (
why is the actual band size different from the predicted?
Block membrane with 5% non-fat milk in PBS-T for 1 hour
at room temperature or longer at 4°C
Incubate membrane with ab26864 at 1/2000 dilution with 1% milk in PBS-T at RT for 1 hour
Rinse 3 times with PBS-T, then wash membrane 3 times for 5 mins
Incubate with HRP-conjugated goat anti IgY/Fc at 1/1000 dilution for with 1% milk PBS-T at RT for 1 hour
Rinse 3 times with PBS-T, then wash 3 times for 5 mins with shaking, before ECL detection
has not yet been referenced specifically in any publications.
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