概述

  • 产品名称
  • 描述
    山羊多克隆抗体to CD30
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Human
  • 免疫原

    Recombinant mouse extracellular domain, expressed in mouse NSO cells. The extracellular domain is fused to the carboxyterminal 6X histidine-tagged Fc portion of human IgG1 by a polypeptide linker.

  • 常规说明
    Endotoxin: <10 ng/mg antibody determined by the LAL method.

性能

应用

Our Abpromise guarantee covers the use of ab10957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    ELISA: Use at a concentration of 0.5 - 1 µg/ml.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml; detects mouse CD30 at approximately 5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. Reduced mouse CD30/Fc monomer has a calculated molecular mass of approximately 55 kDa. Due to glycosylation, the recombinant protein migrates as an approximately 90-100 kDa protein in SDS-PAGE under reducing conditions.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
    • 序列相似性
      Contains 6 TNFR-Cys repeats.
    • 翻译后修饰
      Phosphorylated on serine and tyrosine residues.
    • 细胞定位
      Cytoplasm and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 30 antibody
      • CD30 antibody
      • CD30 antigen antibody
      • CD30L receptor antibody
      • Cytokine receptor CD30 antibody
      • D1S166E antibody
      • KI 1 antibody
      • KI 1 antigen antibody
      • Ki-1 antigen antibody
      • KI1 antibody
      • Lymphocyte activation antigen CD30 antibody
      • TNFRSF 8 antibody
      • Tnfrsf8 antibody
      • TNR8_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 8 antibody
      see all

    实验方案

    文献

    ab10957 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab10957.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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