RabMAb

Anti-CD3 zeta (phospho Y83)抗体[EP776(2)Y] (ab68236)

概述

  • 产品名称Anti-CD3 zeta (phospho Y83)抗体[EP776(2)Y]
    参阅全部 CD3 zeta 一抗
  • 描述
    兔单克隆抗体[EP776(2)Y] to CD3 zeta (phospho Y83)
  • 特异性This antibody detects CD3 zeta phosphorylated on tyrosine 83.
  • 经测试应用适用于: ICC/IF, WB, IPmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD3 zeta (phospho Y83). The exact sequence is proprietary.

  • 阳性对照
    • WB: Jurkat cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP776(2)Y
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab68236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/5000 - 1/10000. Detects a band of approximately 18-22 kDa (predicted molecular weight: 18 kDa).
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 功能Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
    • 疾病相关Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
    • 序列相似性Belongs to the CD3Z/FCER1G family.
      Contains 3 ITAM domains.
    • 结构域The ITAM domains mediate interaction with SHB.
    • 翻译后修饰Phosphorylated on Tyr residues after T-cell receptor triggering.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • CD247 antibody
      • CD247 antigen antibody
      • Cd247 molecule antibody
      • Cd3 antibody
      • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
      • CD3 antigen, zeta subunit antibody
      • CD3-eta antibody
      • CD3H antibody
      • CD3Q antibody
      • Cd3z antibody
      • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
      • CD3Z_HUMAN antibody
      • CD3zeta antibody
      • CD3zeta chain antibody
      • MGC140430 antibody
      • T cell receptor T3 zeta chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 zeta chain antibody
      • T-cell surface glycoprotein CD3 zeta chain antibody
      • T3z antibody
      • TCR zeta chain antibody
      • TCRk antibody
      • TCRZ antibody
      • TCRzeta antibody
      see all

    Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] 图像

    • All lanes : Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab68236) at 1/2000 dilution

      Lane 1 : Untreated Jurkat cells whole cell lysates
      Lane 2 : Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates
      Lane 3 : Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa


      Exposure time : 3 minutes

      Blocking buffer 5% NFDM/TBST

      Diluting buffer 5% NFDM/TBST

    • All lanes : Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab68236) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate, untreated.
      Lane 2 : Jurkat cell lysate, treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 18-22 kDa (why is the actual band size different from the predicted?)
    • Immunocytochemistry/Immunofluorescence analysis of Jurkat cells (untreated, Per treated and Per+LP treated) labelling CD3 zeta (phospho Y83) with ab68236 (left) and CD3 zeta with ab40804 (right) both at a dilution of 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      The image shows increased cytoplamic staining after Pervanadate (1 mM, 30 min) treatment on Jurkat cells. The LP treatment decreased the cytoplasmic staining caused by Pervanadate.

      ab40804 was used as a Pan control for ab68236. The results showed cytoplamic staining on untreated, pervanadate (1 mM, 30 min) treated and Per+LP treated Jurkat cells.

    • Dot blot analysis of CD3 zeta (pY83) phospho peptide (lane 1) and CD3 zeta non-phospho peptide (lane 2) labelling CD3 zeta (phospho Y83) with ab68236 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab68236)参考文献

    This product has been referenced in:
    • Chang YH  et al. A chimeric receptor with NKG2D specificity enhances natural killer cell activation and killing of tumor cells. Cancer Res 73:1777-86 (2013). Read more (PubMed: 23302231) »
    • Smith X  et al. The chemokine CXCL12 generates costimulatory signals in T cells to enhance phosphorylation and clustering of the adaptor protein SLP-76. Sci Signal 6:ra65 (2013). Read more (PubMed: 23901140) »

    See all 4 Publications for this product

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