Anti-CD3 zeta抗体[H146-968] (FITC) (ab91493)


  • 产品名称Anti-CD3 zeta抗体[H146-968] (FITC)
    参阅全部 CD3 zeta 一抗
  • 描述
    亚美尼亚仓鼠单克隆抗体[H146-968] to CD3 zeta (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Rabbit, Cow, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 151-164 of Mouse CD3 zeta

  • 常规说明

    Purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Size exclusion
  • 克隆单克隆
  • 克隆编号H146-968
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab91493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use at a concentration of 2 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
    • 疾病相关Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
    • 序列相似性Belongs to the CD3Z/FCER1G family.
      Contains 3 ITAM domains.
    • 结构域The ITAM domains mediate interaction with SHB.
    • 翻译后修饰Phosphorylated on Tyr residues after T-cell receptor triggering.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • CD247 antibody
      • CD247 antigen antibody
      • Cd247 molecule antibody
      • Cd3 antibody
      • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
      • CD3 antigen, zeta subunit antibody
      • CD3-eta antibody
      • CD3H antibody
      • CD3Q antibody
      • Cd3z antibody
      • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
      • CD3Z_HUMAN antibody
      • CD3zeta antibody
      • CD3zeta chain antibody
      • MGC140430 antibody
      • T cell receptor T3 zeta chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 zeta chain antibody
      • T-cell surface glycoprotein CD3 zeta chain antibody
      • T3z antibody
      • TCR zeta chain antibody
      • TCRk antibody
      • TCRZ antibody
      • TCRzeta antibody
      see all

    Anti-CD3 zeta antibody [H146-968] (FITC) (ab91493)参考文献

    ab91493 has not yet been referenced specifically in any publications.

    Product Wall

    Application Flow Cytometry
    Sample Chicken Cell (PBMC)
    Permeabilization Yes - BD Fix/Perm
    Gating Strategy Lymphocytes/Singlets/CD3/CD3zeta
    Specification PBMC
    Preparation Cell harvesting/tissue preparation method: Ficoll
    Sample buffer: PBS/5% FCS
    Fixation BD Fix/Perm

    Stig Henrik Andersen

    Verified customer

    提交于 May 19 2017

    We do not have any more of ab11505 in stock. Are you able to use the PE-conjugated version of the same clone, ab33395, or the unconjugated version, ab11218? Alternatively, we have a FITC-conjugated hamster monoclonal antibody that is raised against mou...

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    Thank you for your reply.

    Unfortunately, I am sorry to confirm that I have checked the CD3 zeta antibodies that have been tested in flow cytometry and human samples. These have epitopes in the cytoplasmic domain, and so would require permeab...

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    Thank you for your enquiry.

    I have done some literature searching and am not able to find any information to indicate that the membrane and cytoplasmic forms of CD3 Zeta are different, apart from the localization. Therefore, I would suggest ...

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    Thank you for your response.

    ab97077 is a goat anti-rabbit secondary so it is unlikely to react with ab91493 (host species hamster). However, ab97037 is goat anti-mouse secondary antibody and it may cross-react with hamster IgG since mouse an...

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    Thank you for your enquiry.

    Could you please confirm if the customer would like to perform double or triple staining? I am a bit confused why the two selected secondary antibodies ie ab97037 and ab97077 (Cy5 ®) have the same fluorophore? ...

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