Anti-CD3 zeta抗体[H146-968] (ab119827)

概述

  • 产品名称Anti-CD3 zeta抗体[H146-968]
    参阅全部 CD3 zeta 一抗
  • 描述
    亚美尼亚仓鼠单克隆抗体[H146-968] to CD3 zeta
  • 经测试应用适用于: WB, IP, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Chicken, Pig
  • 免疫原

    Synthetic peptide:

    DTYDALHMQTLAPR

    , corresponding to amino acids 151-164 of Mouse CD3 zeta.

  • 阳性对照
    • Mouse splenocyte lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.40
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明ab119827 is purified from cell culture supernatant by protein A affinity chromatography and 0.2 µm filter sterilized. Purity is > 95% by SDS-PAGE.
  • 克隆单克隆
  • 克隆编号H146-968
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab119827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa.
IP Use at an assay dependent concentration.
ICC Use a concentration of 4 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab18479-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
  • 疾病相关Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
  • 序列相似性Belongs to the CD3Z/FCER1G family.
    Contains 3 ITAM domains.
  • 结构域The ITAM domains mediate interaction with SHB.
  • 翻译后修饰Phosphorylated on Tyr residues after T-cell receptor triggering.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • CD247 antibody
    • CD247 antigen antibody
    • Cd247 molecule antibody
    • Cd3 antibody
    • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
    • CD3 antigen, zeta subunit antibody
    • CD3-eta antibody
    • CD3H antibody
    • CD3Q antibody
    • Cd3z antibody
    • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
    • CD3Z_HUMAN antibody
    • CD3zeta antibody
    • CD3zeta chain antibody
    • MGC140430 antibody
    • T cell receptor T3 zeta chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 zeta chain antibody
    • T-cell surface glycoprotein CD3 zeta chain antibody
    • T3z antibody
    • TCR zeta chain antibody
    • TCRk antibody
    • TCRZ antibody
    • TCRzeta antibody
    see all

Anti-CD3 zeta antibody [H146-968] 图像

  • Anti-CD3 zeta antibody [H146-968] (ab119827) at 1 µg/ml + Mouse splenocyte lysate

    Performed under reducing conditions.

    Predicted band size : 19 kDa

Anti-CD3 zeta antibody [H146-968] (ab119827)参考文献

This product has been referenced in:
  • Iida T  et al. Regulation of cell surface expression of CTLA-4 by secretion of CTLA-4-containing lysosomes upon activation of CD4+ T cells. J Immunol 165:5062-8 (2000). Read more (PubMed: 11046036) »
  • Kosugi A  et al. Translocation of tyrosine-phosphorylated TCRzeta chain to glycolipid-enriched membrane domains upon T cell activation. Int Immunol 11:1395-401 (1999). Read more (PubMed: 10464160) »

See all 4 Publications for this product

Product Wall

Thank you for your reply.

Unfortunately, I am sorry to confirm that I have checked the CD3 zeta antibodies that have been tested in flow cytometry and human samples. These have epitopes in the cytoplasmic domain, and so would require permeab...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"