Anti-CD3 zeta抗体[H146-968] - BSA and Azide free (ab119827)
Key features and details
- Armenian hamster monoclonal [H146-968] to CD3 zeta - BSA and Azide free
- Suitable for: ICC, IP, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-CD3 zeta抗体[H146-968] - BSA and Azide free
参阅全部 CD3 zeta 一抗 -
描述
亚美尼亚仓鼠单克隆抗体[H146-968] to CD3 zeta - BSA and Azide free -
宿主
Armenian hamster -
经测试应用
适用于: ICC, IP, WB, Flow Cytmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Chicken, Pig -
免疫原
Synthetic peptide corresponding to Mouse CD3 zeta aa 150-250.
Database link: P20963 -
阳性对照
- WB: Murine splenocytes.
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常规说明
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab119827 is purified from cell culture supernatant by protein A affinity chromatography and 0.2 µm filter sterilized. Purity is > 95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
H146-968 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119827于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC | (1) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 19 kDa.
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Flow Cyt |
Use at an assay dependent concentration.
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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ICC
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 19 kDa. |
Flow Cyt
Use at an assay dependent concentration. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. -
疾病相关
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. -
序列相似性
Belongs to the CD3Z/FCER1G family.
Contains 3 ITAM domains. -
结构域
The ITAM domains mediate interaction with SHB. -
翻译后修饰
Phosphorylated on Tyr residues after T-cell receptor triggering. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 395911 Chicken
- Entrez Gene: 919 Human
- Entrez Gene: 12503 Mouse
- Entrez Gene: 397302 Pig
- Entrez Gene: 100008617 Rabbit
- Entrez Gene: 25300 Rat
- Omim: 186780 Human
- SwissProt: P20963 Human
see all -
别名
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
图片
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All lanes : Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (ab119827)
Lane 1 : Murine splenocytes
Lane 2 : MOLT-4 cell line
Secondary
Lane 1 : conjugated goat-anti-armenian hamster
Lane 2 : Alexa Fluor® 790-conjugated goat-anti-armenian hamster
Performed under reducing conditions.
Predicted band size: 19 kDa -
Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (ab119827) at 2 µg/ml + Murine Splenocytes
Performed under reducing conditions.
Predicted band size: 19 kDa
实验方案
数据表及文件
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Datasheet download
文献 (7)
ab119827 被引用在 7 文献中.
- Lei X et al. A Pan-Histone Deacetylase Inhibitor Enhances the Antitumor Activity of B7-H3-Specific CAR T Cells in Solid Tumors. Clin Cancer Res 27:3757-3771 (2021). PubMed: 33811153
- Wei J et al. PD-1 silencing impairs the anti-tumor function of chimeric antigen receptor modified T cells by inhibiting proliferation activity. J Immunother Cancer 7:209 (2019). PubMed: 31391096
- Jelencic V et al. NK cell receptor NKG2D sets activation threshold for the NCR1 receptor early in NK cell development. Nat Immunol 19:1083-1092 (2018). PubMed: 30224819
- Iida T et al. Regulation of cell surface expression of CTLA-4 by secretion of CTLA-4-containing lysosomes upon activation of CD4+ T cells. J Immunol 165:5062-8 (2000). PubMed: 11046036
- Kosugi A et al. Translocation of tyrosine-phosphorylated TCRzeta chain to glycolipid-enriched membrane domains upon T cell activation. Int Immunol 11:1395-401 (1999). PubMed: 10464160
- Bäckström BT et al. Signaling efficiency of the T cell receptor controlled by a single amino acid in the beta chain constant region. J Exp Med 186:1933-8 (1997). PubMed: 9382891
- Rozdzial MM et al. Developmental regulation of the TCR zeta-chain. Differential expression and tyrosine phosphorylation of the TCR zeta-chain in resting immature and mature T lymphocytes. J Immunol 153:1563-80 (1994). PubMed: 7519206