RabMAb

Anti-CD3 zeta抗体[EP286Y] (ab40804)

概述

  • 产品名称Anti-CD3 zeta抗体[EP286Y]
    参阅全部 CD3 zeta 一抗
  • 描述
    兔单克隆抗体[EP286Y] to CD3 zeta
  • 特异性ab40804 recognises CD3 zeta.
  • 经测试应用适用于: ICC/IF, Flow Cytmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD3 zeta aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • ICC/IF: Jurkat cells. Flow Cyt: Human peripheral blood lymphocytes.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody. 

    Alternative versions available:
    Anti-CD3 zeta antibody (Alexa Fluor® 488) [EP286Y] (ab197532)
    Anti-CD3 zeta antibody (Alexa Fluor® 647) [EP286Y] (ab197037)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP286Y
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/250 - 1/500.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
    • 疾病相关Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
    • 序列相似性Belongs to the CD3Z/FCER1G family.
      Contains 3 ITAM domains.
    • 结构域The ITAM domains mediate interaction with SHB.
    • 翻译后修饰Phosphorylated on Tyr residues after T-cell receptor triggering.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • CD247 antibody
      • CD247 antigen antibody
      • Cd247 molecule antibody
      • Cd3 antibody
      • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
      • CD3 antigen, zeta subunit antibody
      • CD3-eta antibody
      • CD3H antibody
      • CD3Q antibody
      • Cd3z antibody
      • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
      • CD3Z_HUMAN antibody
      • CD3zeta antibody
      • CD3zeta chain antibody
      • MGC140430 antibody
      • T cell receptor T3 zeta chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 zeta chain antibody
      • T-cell surface glycoprotein CD3 zeta chain antibody
      • T3z antibody
      • TCR zeta chain antibody
      • TCRk antibody
      • TCRZ antibody
      • TCRzeta antibody
      see all

    Anti-CD3 zeta antibody [EP286Y] 图像

    • Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling CD3 zeta with ab40804 at a dilution 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were used.

      Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Human peripheral blood lymphocytes stained with ab40804 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab40804, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

    Anti-CD3 zeta antibody [EP286Y] (ab40804)参考文献

    ab40804 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply.

    Unfortunately, I am sorry to confirm that I have checked the CD3 zeta antibodies that have been tested in flow cytometry and human samples. These have epitopes in the cytoplasmic domain, and so would require permeab...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"