概述

  • 产品名称Anti-CD3 zeta抗体
    参阅全部 CD3 zeta 一抗
  • 描述
    兔多克隆抗体to CD3 zeta
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Pig
  • 免疫原

    Synthetic peptide:

    DGILFIYGVILTALFLRVK

    , corresponding to amino acids 36-54 of Human CD3 zeta.

  • 阳性对照
    • Tonsil

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多��隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab17061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol with 10 mM Citrate buffer, pH 6.0.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
    • 疾病相关Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
    • 序列相似性Belongs to the CD3Z/FCER1G family.
      Contains 3 ITAM domains.
    • 结构域The ITAM domains mediate interaction with SHB.
    • 翻译后修饰Phosphorylated on Tyr residues after T-cell receptor triggering.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • CD247 antibody
      • CD247 antigen antibody
      • Cd247 molecule antibody
      • Cd3 antibody
      • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
      • CD3 antigen, zeta subunit antibody
      • CD3-eta antibody
      • CD3H antibody
      • CD3Q antibody
      • Cd3z antibody
      • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
      • CD3Z_HUMAN antibody
      • CD3zeta antibody
      • CD3zeta chain antibody
      • MGC140430 antibody
      • T cell receptor T3 zeta chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 zeta chain antibody
      • T-cell surface glycoprotein CD3 zeta chain antibody
      • T3z antibody
      • TCR zeta chain antibody
      • TCRk antibody
      • TCRZ antibody
      • TCRzeta antibody
      see all

    Anti-CD3 zeta antibody 图像

    • IHC image of ab17061 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17061, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-CD3 zeta antibody (ab17061)参考文献

    This product has been referenced in:
    • Guzik TJ  et al. Role of the T cell in the genesis of angiotensin II induced hypertension and vascular dysfunction. J Exp Med 204:2449-60 (2007). Read more (PubMed: 17875676) »

    See 1 Publication for this product

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    Unseres Wissens nach wurde ab17061 bisher noch nicht inIHC-Fr getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angeb...

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