Anti-CD3 epsilon抗体[PPT3] (PE/Cy5®) (ab25535)


  • 产品名称
    Anti-CD3 epsilon抗体[PPT3] (PE/Cy5®)
    参阅全部 CD3 epsilon 一抗
  • 描述
    小鼠单克隆抗体[PPT3] to CD3 epsilon (PE/Cy5®)
  • 偶联物
    PE/Cy5®. Ex: 496nm, Em: 670nm
  • 经测试应用
    适用于: Cellular Activation, Flow Cyt, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Pig
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 常规说明
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Porcine CD3e is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. ab25535 PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 



Our Abpromise guarantee covers the use of ab25535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Cellular Activation Use at an assay dependent concentration.
Flow Cyt Use 0.2µg for 106 cells. ab67435-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • 功能
    The CD3 complex mediates signal transduction.
  • 序列相似性
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all


This product has been referenced in:
  • Walsh MC  et al. Effects of feeding Bt MON810 maize to pigs for 110 days on peripheral immune response and digestive fate of the cry1Ab gene and truncated Bt toxin. PLoS One 7:e36141 (2012). Flow Cyt ; Pig . Read more (PubMed: 22574138) »
  • Yang H  et al. Preparation of monoclonal anti-porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes. Immunology 88:577-85 (1996). Read more (PubMed: 8881760) »

See all 2 Publications for this product


Thank you for your inquiry.

We do not have any information available about details of the immunogen of this clone of antibody.

We did not perform epitope mapping.

But I can confirm that the epitope is extracellular, becaus...

Read More

Thank you for contacting us. The functional effects of the CD3 epsilon antibody ab25535 clone PPT3 are described in the reference PMID:8881760 (see attached). I hope this helps, please let me know if you need any additional information.