存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
存储溶液 pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS
Concentration information loading...
纯度 IgG fraction
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Use a concentration of 5 µg/ml.
功能 The CD3 complex mediates signal transduction.
序列相似性 Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 ITAM domain.
Information by UniProt
CD3 epsilon antibody
CD3e antigen epsilon polypeptide (TiT3 complex) antibody
Anti-CD3 epsilon antibody [mAbcam97707] 图像
Western blot - CD3 epsilon antibody [mAbcam97707] (ab97707)
All lanes : Anti-CD3 epsilon antibody [mAbcam97707] (ab97707) at 5 µg/ml Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Human thymus tissue lysate - total protein ( ab30146) Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane. Secondary Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 23 kDa Observed band size : 23 kDa Exposure time : 8 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [mAbcam97707] (ab97707)
IHC image of ab97707 staining CD3 epsilon in human normal tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97707, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Anti-CD3 epsilon antibody [mAbcam97707] (ab97707)参考文献
has not yet been referenced specifically in any publications.
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