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RabMAb

Anti-CD3 epsilon抗体[E292] (ab32327)

概述

  • 产品名称
    Anti-CD3 epsilon抗体[E292]
    参阅全部 CD3 epsilon 一抗
  • 描述
    兔单克隆抗体[E292] to CD3 epsilon
  • 宿主
    Rabbit
  • 特异性
    ab32327 recognises CD3 espilon. The antibody does not cross-react with other subunits of CD3.
  • 经测试应用
    适用于: WB, ICC, Flow Cytmore details
    不适用于: IHC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human CD3 epsilon. The exact sequence is proprietary.

  • 表位
    ab32327 reacts with an epitope located in the cytoplasmic region of CD3 epsilon.
  • 阳性对照
    • Jurkat cell lysate
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC 1/50.
Flow Cyt Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for IHC or IP.
  • 靶标

    • 功能
      The CD3 complex mediates signal transduction.
    • 序列相似性
      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    图片

    • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CD3 epsilon with unpurified ab32327 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Anti-CD3 epsilon antibody [E292] (ab32327) at 1/5000 dilution + Jurkat cell lysate

      Predicted band size: 23 kDa
      Observed band size: 23 kDa

    文献

    ab32327 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Vielen dank fuer Ihren Anruf. Ich habe nun ein paar Antikoerper fuer Sie herrausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und fuer IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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