生物素Anti-CD3 epsilon抗体[C363.29B (YCD3-1)] (ab25688)
Key features and details
- Biotin Rat monoclonal [C363.29B (YCD3-1)] to CD3 epsilon
- Suitable for: IP, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2b
概述
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产品名称
生物素Anti-CD3 epsilon抗体[C363.29B (YCD3-1)]
参阅全部 CD3 epsilon 一抗 -
描述
生物素大鼠单克隆抗体[C363.29B (YCD3-1)] to CD3 epsilon -
宿主
Rat -
偶联物
Biotin -
特异性
In the presence of Fc receptor bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. -
经测试应用
适用于: IP, Flow Cyt, Functional Studiesmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to CD3 epsilon. IL-4 producing Th2 cell lines including D10
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表位
This antibody recognises an epitope on the 25kDa epsilon chain of the CD3/TCR complex. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Affinity purified -
克隆
单克隆 -
克隆编号
C363.29B (YCD3-1) -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25688于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use 3µg for 106 cells.
ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
Functional Studies |
Use at an assay dependent concentration.
In vitro depletion of CD3+ cells & In vitro activation of T cells (accessory cells must be provided). |
说明 |
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IP
Use at an assay dependent concentration. |
Flow Cyt
Use 3µg for 106 cells. ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Functional Studies
Use at an assay dependent concentration. In vitro depletion of CD3+ cells & In vitro activation of T cells (accessory cells must be provided). |
靶标
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功能
The CD3 complex mediates signal transduction. -
序列相似性
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 12501 Mouse
- SwissProt: P22646 Mouse
- Unigene: 210361 Mouse
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别名
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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