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The details of the immunogen for this antibody are not available.
In the presence of Fc receptor-bearing accessory cells, soluble ab25278 can activate primed and naïve T cell in vitro.
Our Abpromise guarantee covers the use of ab25278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|IP||Use at an assay dependent concentration.|
|Cellular Activation||Use at an assay dependent concentration.
In vitro cellular activation of T cells. Accessory cells must be provided.
|Functional Studies||Use at an assay dependent concentration.
In vitro depletion of CD3 positive cells.
Flow cytometric analysis of BALB/c mouse splenocytes labelling CD3 epsilon with ab25278 at 0.3 μg/106 cells and labelling CD19 with a Rat Anti-Mouse CD19-FITC antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"