Anti-CD3 epsilon抗体[C363.29B] (Phycoerythrin) (ab25380)

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概述

  • 产品名称
    Anti-CD3 epsilon抗体[C363.29B] (Phycoerythrin)
    参阅全部 CD3 epsilon 一抗
  • 描述
    大鼠单克隆抗体[C363.29B] to CD3 epsilon (Phycoerythrin)
  • 宿主
    Rat
  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性
    ab25380 recognises CD3 epsilon.
  • 经测试应用
    适用于: Flow Cyt, IP, Functional Studies, Cellular Activationmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 表位
    The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
  • 常规说明


    In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.

性能

应用

Our Abpromise guarantee covers the use of ab25380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

ab25380 can be used for in vitro depletion of CD3 positive cells.
Cellular Activation Use at an assay dependent concentration.

ab25380 can activate primed and naive T cell in vitro (accessory cells must be provided).

靶标

  • 功能
    The CD3 complex mediates signal transduction.
  • 序列相似性
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    图片

    • Flow Cytometry - Anti-CD3 epsilon antibody [C363.29B] (Phycoerythrin) (ab25380)
      Flow Cytometry - Anti-CD3 epsilon antibody [C363.29B] (Phycoerythrin) (ab25380)

      BALB/c lymph node cells were double stained with ab25380 and with anti-IL2RB (ab24943) at 1µg/106 cells. Small lymphocytes were gated and analyzed on a flow cytometer.

    文献

    This product has been referenced in:
    • Tarrant TK  et al. G protein-coupled receptor kinase-3-deficient mice exhibit WHIM syndrome features and attenuated inflammatory responses. J Leukoc Biol 94:1243-51 (2013). Mouse . Read more (PubMed: 23935208) »

    See 1 Publication for this product

    发表研究结果有使用 ab25380？请让我们知道，以便我们可以引用本数据表中的参考文章。

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