The details of the immunogen for this antibody are not available.
In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.
Our Abpromise guarantee covers the use of ab25380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | Use 1µg for 106 cells. | |
IP | Use at an assay dependent concentration. | |
Functional Studies | Use at an assay dependent concentration. ab25380 can be used for in vitro depletion of CD3 positive cells. |
|
Cellular Activation | Use at an assay dependent concentration. ab25380 can activate primed and naive T cell in vitro (accessory cells must be provided). |
BALB/c lymph node cells were double stained with ab25380 and with anti-IL2RB (ab24943) at 1µg/106 cells. Small lymphocytes were gated and analyzed on a flow cytometer.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"