Anti-CD3 epsilon抗体[C363.29B] (PE/Cy5®) (ab25531)

概述

  • 产品名称
    Anti-CD3 epsilon抗体[C363.29B] (PE/Cy5®)
    参阅全部 CD3 epsilon 一抗
  • 描述
    大鼠单克隆抗体[C363.29B] to CD3 epsilon (PE/Cy5®)
  • 偶联物
    PE/Cy5®. Ex: 496nm, Em: 670nm
  • 经测试应用
    适用于: Flow Cyt, IPmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 表位
    This antibody recognises an epitope on the 25kDa epsilon chain of the CD3/TCR complex.
  • 常规说明
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.


    In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining and is a good immunoprecipitating antibody. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, 0.20% Gelatin, PBS and stabilising agent.
  • Concentration information loading...
  • 纯度
    IgG fraction
  • Primary antibody说明
    In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining and is a good immunoprecipitating antibody. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).
  • 克隆
    单克隆
  • 克隆编号
    C363.29B
  • 同种型
    IgG2c
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.
IP Use at an assay dependent dilution.

靶标

  • 功能
    The CD3 complex mediates signal transduction.
  • 序列相似性
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

图片

  • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (PE/Cy5) ab25531 (1 µg/106cells) and Rat Anti-Mouse CD19-PE.

文献

This product has been referenced in:
  • Carrithers MD  et al. Differential adhesion molecule requirements for immune surveillance and inflammatory recruitment. Brain 123 ( Pt 6):1092-101 (2000). Read more (PubMed: 10825349) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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