PE/Cy5® Anti-CD3 epsilon抗体[C363.29B] (ab25531)
Key features and details
- PE/Cy5® Rat monoclonal [C363.29B] to CD3 epsilon
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG2c
概述
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产品名称
PE/Cy5® Anti-CD3 epsilon抗体[C363.29B]
参阅全部 CD3 epsilon 一抗 -
描述
PE/Cy5®大鼠单克隆抗体[C363.29B] to CD3 epsilon -
宿主
Rat -
偶联物
PE/Cy5®. Ex: 496nm, Em: 670nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Full length protein corresponding to Mouse CD3 epsilon. " IL-4 producing Th2 cell lines including D10"
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表位
This antibody recognises an epitope on the 25kDa epsilon chain of the CD3/TCR complex. -
常规说明
In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% Gelatin, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading...
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纯度
IgG fraction -
Primary antibody说明
In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining and is a good immunoprecipitating antibody. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided). -
克隆
单克隆 -
克隆编号
C363.29B -
同种型
IgG2c -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25531于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 1µg for 106 cells.
|
说明 |
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Flow Cyt
Use 1µg for 106 cells. |
靶标
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功能
The CD3 complex mediates signal transduction. -
序列相似性
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 12501 Mouse
- SwissProt: P22646 Mouse
- Unigene: 210361 Mouse
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别名
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab25531 被引用在 1 文献中.
- Carrithers MD et al. Differential adhesion molecule requirements for immune surveillance and inflammatory recruitment. Brain 123 ( Pt 6):1092-101 (2000). PubMed: 10825349