Anti-CD3 epsilon抗体(ab119332)

概述

  • 产品名称Anti-CD3 epsilon抗体
    参阅全部 CD3 epsilon 一抗
  • 描述
    兔多克隆抗体to CD3 epsilon
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, surrounding amino acids 197-201 (D-L-Y-S-G) of Human CD3 epsilon.

  • 阳性对照
    • MOLT4 and Jurkat cell extracts. IHC-P: FFPE human spleen tissue sections.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab119332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能The CD3 complex mediates signal transduction.
  • 序列相似性Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Anti-CD3 epsilon antibody 图像

  • IHC image of CD3-epsilon staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119332, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-CD3 epsilon antibody (ab119332) at 1/500 dilution

    Lane 1 : MOLT4 cell extract
    Lane 2 : Jurkat cell extract


    Predicted band size : 23 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)

Anti-CD3 epsilon antibody (ab119332)参考文献

ab119332 has not yet been referenced specifically in any publications.

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