Anti-CD3 epsilon抗体[145-2C11] (Phycoerythrin) (ab25665)

概述

  • 产品名称Anti-CD3 epsilon抗体[145-2C11] (Phycoerythrin)
    参阅全部 CD3 epsilon 一抗
  • 描述
    亚美尼亚仓鼠单克隆抗体[145-2C11] to CD3 epsilon (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性This antibody recognises CD3 epsilon.
  • 经测试应用适用于: Flow Cyt, WB, IP, Functional Studiesmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation: H2Kb specific mouse cytotoxic T lymphocyte clone BM10-37.

  • 表位This antibody recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
  • 常规说明


    In the presence of Fc receptor bearing accessory cells, this antibody, in the soluble form, can activate primed and naïve T cell in vitro. This antibody also induces redirected lysis of specific target cells by CTL clones, and can block lysis of specific target cells by antigen specific CTL's. Immobilized ab25665 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab25665 may result in apoptotic cell death.

性能

  • 形式Liquid
  • 存放说明Store at +4°C in the dark. Do not freeze.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明In the presence of Fc receptor bearing accessory cells, this antibody, in the soluble form, can activate primed and naïve T cell in vitro. This antibody also induces redirected lysis of specific target cells by CTL clones, and can block lysis of specific target cells by antigen specific CTL's. Immobilized ab25665 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab25665 may result in apoptotic cell death.
  • 克隆单克隆
  • 克隆编号145-2C11
  • 同种型IgG
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
WB
IP
Functional Studies
  • 应用说明Flow Cyt: Use 1µg for 106 cells.
    FuncS: Use at an assay dependent dilution. Ab25665 can be used for in vitro depletion of CD3 positive cells and for in vitro activation of T cells.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能The CD3 complex mediates signal transduction.
    • 序列相似性Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Anti-CD3 epsilon antibody [145-2C11] (Phycoerythrin) (ab25665)参考文献

    ab25665 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"