Anti-CD3 epsilon抗体[145-2C11] (FITC) (ab24947)

概述

  • 产品名称Anti-CD3 epsilon抗体[145-2C11] (FITC)
    参阅全部 CD3 epsilon 一抗
  • 描述
    亚美尼亚仓鼠单克隆抗体[145-2C11] to CD3 epsilon (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性ab24947 recognises CD3 epsilon chain.
  • 经测试应用适用于: Flow Cyt, IP, WB, Functional Studiesmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation: H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37

  • 阳性对照
    • BALB/c mouse spleen cells.
  • 常规说明


    ab24947 can induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen specific CTL’s. Immobilized ab24947 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab24947 may result in apoptotic cell death.

性能

应用

Our Abpromise guarantee covers the use of ab24947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 3µg for 106 cells. ab18473-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
Functional Studies Use at an assay dependent concentration.

In vitro depletion of CD3 positive cells, and for in vitro activation of T cells.

靶标

  • 功能The CD3 complex mediates signal transduction.
  • 序列相似性Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Anti-CD3 epsilon antibody [145-2C11] (FITC) 图像

  • BALB/c spleen cells were double-stained with hamster anti mouse CD3 epsilon FITC conjugated (ab24947) and rat anti mouse CD19 RPE. Lymphocytes were gated and analyzed on a flow cytometer.
  • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [145-2C11] (FITC) ab24947 (1 µg/106 cells), and Rat Anti-Mouse CD19-PE.

  • ab24947 staining mouse BALB/c spleen cells by flow cytometry. 1µg antibody was used for 106 cells.

Anti-CD3 epsilon antibody [145-2C11] (FITC) (ab24947)参考文献

ab24947 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab24947.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"