Anti-CD3 epsilon抗体[145-2C11] (Biotin-maleimide) (ab25172)

概述

  • 产品名称Anti-CD3 epsilon抗体[145-2C11] (Biotin-maleimide)
    参阅全部 CD3 epsilon 一抗
  • 描述
    亚美尼亚仓鼠单克隆抗体[145-2C11] to CD3 epsilon (Biotin-maleimide)
  • 偶联物Biotin-maleimide
  • 特异性ab25172 recognises Mouse CD3 epsilon (Mr 25 kDa).
  • 经测试应用适用于: Functional Studies, Flow Cyt, IP, WB, Cellular Activationmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37.

  • 表位ab25172 recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
  • 常规说明


    In the presence of Fc receptor-bearing accessory cells, soluble ab25172 can activate primed and naïve T cell in vitro and can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized ab25172 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明In the presence of Fc receptor-bearing accessory cells, soluble ab25172 can activate primed and naïve T cell in vitro and can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized ab25172 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.
  • 克隆单克隆
  • 克隆编号145-2C11
  • 同种型IgG
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

In vitro depletion of CD3+ cells.

Flow Cyt Use 3µg for 106 cells.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
Cellular Activation Use at an assay dependent concentration.

In vitro activation of T cells.

靶标

  • 功能The CD3 complex mediates signal transduction.
  • 序列相似性Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Anti-CD3 epsilon antibody [145-2C11] (Biotin-maleimide) 图像

  • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [145-2C11] (Biotin-maleimide) ab25172 (0.3 µg/106 cells), and Rat Anti-Mouse CD19-FITC followed by Streptavidin-PE.

Anti-CD3 epsilon antibody [145-2C11] (Biotin-maleimide) (ab25172)参考文献

This product has been referenced in:
  • Mira E  et al. Statins induce regulatory T cell recruitment via a CCL1 dependent pathway. J Immunol 181:3524-34 (2008). IHC-Fr ; Mouse . Read more (PubMed: 18714025) »
  • Leo O  et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc Natl Acad Sci U S A 84:1374-8 (1987). Read more (PubMed: 2950524) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"