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H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37.
In the presence of Fc receptor-bearing accessory cells, soluble ab25172 can activate primed and naïve T cell in vitro and can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized ab25172 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.
Our Abpromise guarantee covers the use of ab25172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
In vitro depletion of CD3+ cells.
|Flow Cyt||Use 3µg for 106 cells.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.|
|Cellular Activation||Use at an assay dependent concentration.
In vitro activation of T cells.
Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [145-2C11] (Biotin-maleimide) ab25172 (0.3 µg/106 cells), and Rat Anti-Mouse CD19-FITC followed by Streptavidin-PE.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"