概述

  • 产品名称Anti-CD3抗体[SP7]
    参阅全部 CD3 一抗
  • 描述
    兔单克隆抗体[SP7] to CD3
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IHC-P, WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
    预测可用于: Sheep, Rabbit, Horse, Chicken, Cow, Cat, Dog, Baboon, Woodchuck
  • 免疫原

    Synthetic peptide:

    KAKAKPVTRGAGA

    , corresponding to amino acids 156-168 of Human CD3 epsilon chain.

  • 表位aa 156-168 of epsilon chain of Human CD3 protein (intracytoplasmic).
  • 阳性对照
    • Tonsil
  • 常规说明

    This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.

性能

应用

Our Abpromise guarantee covers the use of ab16669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration. PubMed: 18658050
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

WB 1/200. Predicted molecular weight: 23 kDa.
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能The CD3 complex mediates signal transduction.
  • 疾病相关Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • 序列相似性Contains 1 ITAM domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

Anti-CD3 antibody [SP7] 图像

  • Flow cytometric analysis of rabbit anti-CD3 (SP7) antibody ab16669 (1/100) in Jurkats cells (green) compare to negative control of rabbit IgG (blue).

  • Human peripheral blood lymphocytes stained with ab16669 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab16669, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • Immunohistochemical (Formalin / PFA fixed paraffin-embedded sections) staining of of Tonsil tissue labeling CD3 (SP7) with ab16669 at 1/150 dilution.

  • Human tonsil stained with ab16669.
  • ab16669 staining rat infarcted heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Myocardial infarction was produced in a rat model following the ligation of the left anterior descending (LAD) coronary artery. Tissue was harvested 6 w following infarct, fixed with Histochoice for 72 hr, paraffin sectioned and the slide was then baked prior to CD3 staining. ab16669 at 1/200 was incubated overnight at 4°C. The image was taken with a confocal laser scanning microscope and shows cells giving strong inmmunofluorescence staining for CD3 antigen (green), indicating presence of cells of T-lymphocytes origin in the infarct zone of the heart tissue, counterstained nuclei with DAPI (blue). Note, CD3 tended to be present in nests of 2-5 cells that were non-uniformly distributed in the infarct zone. In addition, the image shows that the CD3 localization is predominantly membrane based and to a certain extent intracytoplasmic.

    See Abreview

  • Anti-CD3 antibody [SP7] (ab16669) at 1/25 dilution + Jurkat cell lysate

    Predicted band size : 23 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
  • ab16669 staining CD3 in murine brain tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in acetone, blocked using 5% serum for 30 minutes at 25°C and then incubated with ab16669 at a 1/200 dilution for 18 hours at 4°C. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.

    See Abreview

  • Immunohistochemical analysis of Human tonsil tissue, staining CD3 (green) with ab16669.

    Antigen retrieval was performed by heat mediation in citrate buffer (pH 6) and blocked with 5% goat serum and 5% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. A Cy3®-conjugated anti-rabbit IgG was used as the secondary antibody.

Anti-CD3 antibody [SP7] (ab16669)参考文献

This product has been referenced in:
  • Shiokawa M  et al. Pathogenicity of IgG in patients with IgG4-related disease. Gut N/A:N/A (2016). Human . Read more (PubMed: 26964842) »
  • Kanazawa H  et al. Durable Benefits of Cellular Postconditioning: Long-Term Effects of Allogeneic Cardiosphere-Derived Cells Infused After Reperfusion in Pigs with Acute Myocardial Infarction. J Am Heart Assoc 5:N/A (2016). IHC-P ; Pig . Read more (PubMed: 26857066) »

See all 40 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Dog Cell (skeletal muscle)
Specification skeletal muscle
Permeabilization No
Fixative acetone/methanol 1:1 cold
Username

Dr. Ling Guo

Verified customer

提交于 Feb 10 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Artery)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization No
Specification Artery
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative 10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Sep 13 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Permeabilization Yes - 1% Triton-X
Specification Brain
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% Neutral Buffered Formalin
Username

Abcam user community

Verified customer

提交于 Aug 19 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (lymph node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH 6.0 or TE pH 9.0
Permeabilization No
Specification lymph node
Blocking step (agent) for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. ian beckman

Verified customer

提交于 Jun 03 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Spleen CD3)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: IHC Tek epitope retrieval
Permeabilization No
Specification Spleen CD3
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. Kunal Patel

Verified customer

提交于 May 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6.0
Permeabilization No
Specification Lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. Jim Hsiao

Verified customer

提交于 Mar 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (stomach)
Permeabilization No
Specification stomach
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Mar 10 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bone marrow)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6
Permeabilization No
Specification Bone marrow
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

提交于 Mar 04 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate Cancer)
Antigen retrieval step Heat mediated
Permeabilization No
Specification Prostate Cancer
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 12 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (bladder)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization No
Specification bladder
Blocking step Peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 27°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 12 2015

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