Anti-CD3抗体[KT3] (ab33429)
Key features and details
- Rat monoclonal [KT3] to CD3
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CD3抗体[KT3]
参阅全部 CD3 一抗 -
描述
大鼠单克隆抗体[KT3] to CD3 -
宿主
Rat -
经测试应用
适用于: IHC-Fr, ICC/IF, Flow Cytmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse CD3. mature T cells (Mouse)
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常规说明
ab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
Primary antibody说明
ab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells. -
克隆
单克隆 -
克隆编号
KT3 -
骨髓瘤
NS0 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33429于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
|
Flow Cyt |
Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
图片
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ab33429 staining CD3 in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rat IgG conjugated to Alexa Fluor®488 was used as secondary antibody at a 1/250 dilution.
实验方案
数据表及文件
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Datasheet download
文献 (6)
ab33429 被引用在 6 文献中.
- Luo Z et al. Ex vivo anchored PD-L1 functionally prevent in vivo renal allograft rejection. Bioeng Transl Med 7:e10316 (2022). PubMed: 36176616
- Gao M et al. Poldip2 mediates blood-brain barrier disruption and cerebral edema by inducing AQP4 polarity loss in mouse bacterial meningitis model. CNS Neurosci Ther 26:1288-1302 (2020). PubMed: 32790044
- Viitala M et al. Immunotherapeutic Blockade of Macrophage Clever-1 Reactivates the CD8+ T-cell Response against Immunosuppressive Tumors. Clin Cancer Res N/A:N/A (2019). PubMed: 30755440
- Chung WY et al. An ex vivo porcine spleen perfusion as a model of bacterial sepsis. ALTEX 36:29-38 (2019). PubMed: 30076761
- Yuzhalin AE et al. Proteomics analysis of the matrisome from MC38 experimental mouse liver metastases. Am J Physiol Gastrointest Liver Physiol 317:G625-G639 (2019). PubMed: 31545917
- Kumar L et al. Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function. J Exp Med 211:929-42 (2014). PubMed: 24752297