概述

  • 产品名称
    Anti-CD3抗体[KT3]
    参阅全部 CD3 一抗
  • 描述
    大鼠单克隆抗体[KT3] to CD3
  • 经测试应用
    适用于: ICC/IF, Flow Cyt, IHC-Frmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation mature T cells (Mouse)

  • 常规说明


    ab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells.

性能

应用

Our Abpromise guarantee covers the use of ab33429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/100. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能
      The CD3 complex mediates signal transduction.
    • 疾病相关
      Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    • 序列相似性
      Contains 1 ITAM domain.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • Cd247 antibody
      • CD247 antigen antibody
      • CD247 antigen, zeta subunit antibody
      • CD247 molecule antibody
      • CD3 antibody
      • CD3 antigen, delta subunit antibody
      • CD3 delta antibody
      • CD3 epsilon antibody
      • CD3 eta antibody
      • CD3 gamma antibody
      • CD3 molecule delta polypeptide antibody
      • CD3 molecule, epsilon polypeptide antibody
      • CD3 molecule, gamma polypeptide antibody
      • CD3 zeta antibody
      • CD3-DELTA antibody
      • CD3d antibody
      • CD3D antigen delta polypeptide antibody
      • CD3d antigen, delta polypeptide (TiT3 complex) antibody
      • CD3d molecule delta antibody
      • CD3d molecule delta CD3 TCR complex antibody
      • CD3d molecule, delta (CD3-TCR complex) antibody
      • CD3D_HUMAN antibody
      • CD3E antibody
      • CD3e antigen antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule epsilon CD3 TCR complex antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3epsilon antibody
      • CD3G antibody
      • CD3g antigen antibody
      • CD3G antigen gamma polypeptide antibody
      • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
      • CD3g molecule gamma antibody
      • CD3g molecule gamma CD3 TCR complex antibody
      • CD3g molecule, gamma (CD3-TCR complex) antibody
      • CD3H antibody
      • CD3Q antibody
      • CD3Z antibody
      • CD3zeta antibody
      • Ctg3 antibody
      • FLJ17620 antibody
      • FLJ17664 antibody
      • FLJ18683 antibody
      • FLJ79544 antibody
      • FLJ94613 antibody
      • IMD19 antibody
      • Leu-4 antibody
      • MGC138597 antibody
      • OKT3, delta chain antibody
      • OTTHUMP00000032544 antibody
      • T cell receptor antibody
      • T cell receptor T3 delta chain antibody
      • T cell receptor T3 gamma chain antibody
      • T cell receptor T3 zeta chain antibody
      • T cell receptor zeta chain antibody
      • T cell surface glycoprotein CD3 antibody
      • T cell surface glycoprotein CD3 delta chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T cell surface glycoprotein CD3 gamma chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, delta subunit of T3 antibody
      • T-cell antigen receptor complex, epsilon subunit of T3 antibody
      • T-cell antigen receptor complex, gamma subunit of T3 antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 delta chain antibody
      • T-cell receptor T3 gamma chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 delta chain antibody
      • T-cell surface glycoprotein CD3 gamma chain antibody
      • T3 antibody
      • T3d antibody
      • T3e antibody
      • T3g antibody
      • T3z antibody
      • TCRE antibody
      • TCRk antibody
      • Tcrz antibody
      • TCRzeta antibody
      see all

    Anti-CD3 antibody [KT3] 图像

    • ab33429 staining CD3 in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rabbit IgG conjugated to Alexa Fluor®488 was used as secondary antibody at a 1/250 dilution.

      See Abreview

    Anti-CD3 antibody [KT3] (ab33429)参考文献

    This product has been referenced in:
    • Kumar L  et al. Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function. J Exp Med 211:929-42 (2014). Read more (PubMed: 24752297) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (bone marrow)
    Specification
    bone marrow
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 19 2011

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (thymus)
    Specification
    thymus
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 30 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Spleen)
    Specification
    Spleen
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 30 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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