The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25µg for 105-8 cells. Use a final volume of 100µl.
ab18436-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
The CD3 complex mediates signal transduction.
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Staining of rat splenocytes with anti-CD45R FITC and staining buffer (autofluorescence) (left) or 0.125µg of ab95508 (right) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
Tran GT et al. Reversal of experimental allergic encephalomyelitis with non-mitogenic, non-depleting anti-CD3 mAb therapy with a preferential effect on T(h)1 cells that is augmented by IL-4. Int Immunol13:1109-20 (2001).
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Nicolls MR et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody. Transplantation55:459-68 (1993).
Read more (PubMed: 8456460) »