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Synthetic peptide corresponding to CD3. Synthetic peptide derived from cytoplasmic epitope of CD3.
Our Abpromise guarantee covers the use of ab11089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
Membrane permeabilization is recommended for this application.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1mM EDTA pH8.0 is recommended for this purpose.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 23.1 kDa.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD3 with ab11089. Heat mediated antigen retrieval was performed using EDTA buffer pH 9.0.
Staining of human peripheral blood lymphocytes with Rat anti Human CD3 (ab11089) by Flow cytometry (FACS).
ab11089 staining CD3 in mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 15% serum for 60 minutes at 20°C. Antigen retrieval was by heat mediation in NaCitrate (pH 6). Samples were incubated with primary antibody (1/250 in TBS) for 18 hours at 20°C. An Alexa Flour®conjugated goat anti-rat IgG polyclonal (1/400) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"