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Our Abpromise guarantee covers the use of ab828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50. PubMed: 1839075918390759|
|IHC-Fr||1/10 - 1/50. ABC method.|
This immunoperoxidase picture shows inflammatory cell infiltration with many T-lymphocytes in the interstitium of human kidneys with congenital nephrotic syndrome of the Finnish type (CNF, NPHS1). Immunoperoxidase staining of frozen sections with ab828 antibody (oval shaped, unstained spaces on the left are glomeruli).
This image was kindly supplied as part of the review submitted by Arvi-Matti-Kuusniemi.
Ab828 positively staining formaldehyde fixed human tonsil tissue at 1/50 in conjunction with goat anti rabbit HRP. Detection by DAB.
Pressure cooker heat mediated antigen retrieval was employed in citrate buffer pH 6.0.
This image is courtesy of an Abreview submitted by Melanie Black on 22 Aug 05. We do not have any further information relating to this image.
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