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Our Abpromise guarantee covers the use of ab32899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).|
ab32899 (5µg/ml) shows staining of CD2BP2 in paraffin embedded Human Spleen following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows nucleolar staining in select splenocytes.
ab32899 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"