概述
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产品名称Anti-CD2BP2抗体
参阅全部 CD2BP2 一抗 -
描述山羊多克隆抗体to CD2BP2
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宿主Goat
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经测试应用适用于: IHC-P, ELISA, WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Chimpanzee
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免疫原
Synthetic peptide:
PKRKVTFQGVGDE
, corresponding to N terminal amino acids 2-14 of Human CD2BP2. -
阳性对照
- W: Human lymph node, thymus and tonsil lysates.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, 0.5mg/ml Tris saline. pH 7.3. -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab32899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ELISA | 1/32000. | |
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa). |
靶标
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功能Involved in pre-mRNA splicing as component of the U5 snRNP complex that is involved in spliceosome assembly.
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序列相似性Contains 1 GYF domain.
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细胞定位Cytoplasm. Nucleus. Predominantly nuclear.
- Information by UniProt
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数据库链接
- Entrez Gene: 10421 Human
- Entrez Gene: 70233 Mouse
- Entrez Gene: 293505 Rat
- Omim: 604470 Human
- SwissProt: O95400 Human
- SwissProt: Q9CWK3 Mouse
- Unigene: 202677 Human
- Unigene: 28050 Mouse
see all -
别名
- CD2 (cytoplasmic tail) binding protein 2 antibody
- CD2 antigen (cytoplasmic tail) binding protein 2 antibody
- CD2 antigen cytoplasmic tail-binding protein 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2BP2 antibody (ab32899)ab32899 (5µg/ml) shows staining of CD2BP2 in paraffin embedded Human Spleen following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows nucleolar staining in select splenocytes.
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Western blot - Anti-CD2BP2 antibody (ab32899)Anti-CD2BP2 antibody (ab32899) at 0.3 µg/ml + 35ug of human thymus lysate in RIPA buffer
Predicted band size: 38 kDa
Observed band size: 50 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
Detected by chemiluminescence.
文献
ab32899 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"