概述

  • 产品名称
  • 描述
    兔多克隆抗体to CD2AP
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A peptide from the C terminal residues of human CD2AP.

  • 阳性对照
    • Human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab84829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100 - 1/500.

靶标

  • 功能
    Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.
  • 组织特异性
    Widely expressed in fetal and adult tissues.
  • 疾病相关
    Defects in CD2AP are the cause of susceptibility to focal segmental glomerulosclerosis type 3 (FSGS3) [MIM:607832]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • 序列相似性
    Contains 3 SH3 domains.
  • 结构域
    The Pro-rich domain may mediate binding to SH3 domains.
    Potential homodimerization is mediated by the coiled coil domain.
  • 翻译后修饰
    Phosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src.
  • 细胞定位
    Cytoplasm > cytoskeleton. Cell projection > ruffle. Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles. Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adapter protein CMS antibody
    • AL024079 antibody
    • C78928 antibody
    • Cas ligand with multiple SH3 domains antibody
    • CD2 associated protein antibody
    • CD2-associated protein antibody
    • CD2AP antibody
    • CD2AP_HUMAN antibody
    • CMS antibody
    • Mesenchyme to epithelium transition protein with SH3 domains 1 antibody
    • METS 1 antibody
    • Mets1 antibody
    see all

图片

  • ab84829 at a 1/200 dilution showing cytoplasmic staining of CD2AP in formalin-fixed, paraffin-embedded kidney tissue sections by immunohistochemistry.
  • ICC/IF image of ab84829 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84829, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Huang G  et al. Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathway. Int J Mol Med 39:559-568 (2017). Human . Read more (PubMed: 28112381) »

See 1 Publication for this product

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