概述

  • 产品名称Anti-CD299抗体
    参阅全部 CD299 一抗
  • 描述
    兔多克隆抗体to CD299
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Pig, Chimpanzee, African Green Monkey
  • 免疫原

    A region within synthetic peptide: SKVPSSLSQE QSEQDAIYQN LTQLKAAVGE LSEKSKLQEI YQELTQLKAA, corresponding to N terminal amino acids 73-122 of Human CD299

  • 阳性对照
    • HepG2 cell lysate; human kidney tissue

性能

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应用

Our Abpromise guarantee covers the use of ab58603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus.
  • 组织特异性Predominantly highly expressed in liver sinusoidal endothelial cells and in lymph node. Found in placental endothelium but not in macrophages. Expressed in type II alveolar cells and lung endothelial cells.
  • 序列相似性Contains 1 C-type lectin domain.
  • 结构域The tandem repeat domain, also called neck domain, mediates oligomerization.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C type lectin domain family 4, member M antibody
    • C-type lectin domain family 4 member M antibody
    • CD209 antigen like antibody
    • CD209 antigen like protein 1 antibody
    • CD209 antigen-like protein 1 antibody
    • CD209L antibody
    • CD209L1 antibody
    • CD299 antibody
    • CD299 antigen antibody
    • CLC4M_HUMAN antibody
    • CLEC4M antibody
    • DC SIGN related protein antibody
    • DC SIGN2 antibody
    • DC SIGNR antibody
    • DC-SIGN-related protein antibody
    • DC-SIGN2 antibody
    • DC-SIGNR antibody
    • DCSIGN related protein antibody
    • DCSIGNR antibody
    • Dendritic cell-specific ICAM-3-grabbing non-integrin 2 antibody
    • HP10347 antibody
    • L SIGN antibody
    • L-SIGN antibody
    • Liver/lymph node specific ICAM3 grabbing nonintegrin antibody
    • Liver/lymph node-specific ICAM-3-grabbing non-integrin antibody
    • LSIGN antibody
    • Mannose binding C type lectin DC SIGNR antibody
    • MGC129964 antibody
    • MGC47866 antibody
    see all

Anti-CD299 antibody 图像

  • Anti-CD299 antibody (ab58603) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD299 with ab58603 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • ICC/IF image of ab58603 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58603, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CD299 antibody (ab58603)参考文献

This product has been referenced in:
  • Martinez MG  et al. Utilization of human DC-SIGN and L-SIGN for entry and infection of host cells by the New World arenavirus, Junín virus. Biochem Biophys Res Commun 441:612-7 (2013). Human . Read more (PubMed: 24183720) »

See 1 Publication for this product

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