Anti-CD28抗体[CD28.2] - Low endotoxin,Azide free (ab85986)
Key features and details
- Mouse monoclonal [CD28.2] to CD28 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD28抗体[CD28.2] - Low endotoxin,Azide free
参阅全部 CD28 一抗 -
描述
小鼠单克隆抗体[CD28.2] to CD28 - Low endotoxin,Azide free -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Non human primates -
免疫原
Tissue, cells or virus corresponding to CD28. Human CD28 transfected murine T cell hybridoma.
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阳性对照
- Flow Cyt: CD28 positive lymphocytes.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.20
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
Azide free. -
无载体
是 -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. Endotoxin level is less than or equal to 0.01 EU/ug, as determined by the LaL assay. -
克隆
单克隆 -
克隆编号
CD28.2 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- PE Anti-CD28 antibody [CD28.2], prediluted (ab134418)
- FITC Anti-CD28 antibody [CD28.2] (ab134472)
- PerCP/Cy5.5® Anti-CD28 antibody [CD28.2], prediluted (ab157318)
- PE/Cy7® Anti-CD28 antibody [CD28.2] (ab234276)
- Biotin Anti-CD28 antibody [CD28.2] (ab267385)
- APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)
- PerCP Anti-CD28 antibody [CD28.2] (ab88034)
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85986于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use a concentration of 1 - 12 µg/ml.
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说明 |
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Flow Cyt
Use a concentration of 1 - 12 µg/ml. |
靶标
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功能
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
组织特异性
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 940 Human
- Omim: 186760 Human
- SwissProt: P10747 Human
- Unigene: 591629 Human
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别名
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
图片
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Flow cytometry surface staining pattern of human peripheral blood stained using ab85986 GAM APC.
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Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab85986 GAM APC.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab85986 被引用在 11 文献中.
- Liu R et al. Serum-derived exosomes containing NEAT1 promote the occurrence of rheumatoid arthritis through regulation of miR-144-3p/ROCK2 axis. Ther Adv Chronic Dis 12:2040622321991705 (2021). PubMed: 33995991
- Fehrholz M et al. Impact of the New Generation Reconstituted Surfactant CHF5633 on Human CD4+ Lymphocytes. PLoS One 11:e0153578 (2016). PubMed: 27077658
- Urso K et al. NFATc3 regulates the transcription of genes involved in T-cell activation and angiogenesis. Blood 118:795-803 (2011). PubMed: 21642596
- Chen ML et al. Antipsychotic drugs suppress the AKT/NF-?B pathway and regulate the differentiation of T-cell subsets. Immunol Lett 140:81-91 (2011). Functional Studies ; Human . PubMed: 21763349
- Scharschmidt E et al. Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol 24:3860-73 (2004). PubMed: 15082780