Anti-CD276抗体[6A1] (ab105922)

概述

  • 产品名称Anti-CD276抗体[6A1]
    参阅全部 CD276 一抗
  • 描述
    小鼠单克隆抗体[6A1] to CD276
  • 经测试应用适用于: WB, IHC-P, ICC/IF, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified recombinant fragment of Human CD276 expressed in E. Coli.

  • 阳性对照
    • WB: HEK293 cell lysate IHC-P: Human cervical cancer and ovarian cancer tissues FC: PC-3 cells

性能

应用

Our Abpromise guarantee covers the use of ab105922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/1000. Predicted molecular weight: 57 kDa.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
  • 组织特异性Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
  • 序列相似性Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4Ig B7 H3 antibody
    • 4Ig-B7-H3 antibody
    • AU016588 antibody
    • B7 H3 antibody
    • B7 homolog 3 antibody
    • B7-H3 antibody
    • B7H3 antibody
    • B7RP-2 antibody
    • CD_antigen=CD276 antibody
    • CD276 antibody
    • CD276 antigen antibody
    • CD276 molecule antibody
    • CD276_HUMAN antibody
    • Costimulatory molecule antibody
    • Flags: Precursor antibody
    • PSEC0249 antibody
    • UNQ309/PRO352 antibody
    see all

Anti-CD276 antibody [6A1] 图像

  • All lanes : Anti-CD276 antibody [6A1] (ab105922) at 1/200 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Transfected HEK293 cell lysate


    Predicted band size : 57 kDa
  • ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human cervical cancer tissues.
  • ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human ovarian cancer tissues.
  • Flow cytometric analysis of PC-3 cells using ab105922 (green) and negative control (purple).

Anti-CD276 antibody [6A1] (ab105922)参考文献

ab105922 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Specification Prostate cancer
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde
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提交于 Nov 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"