Anti-CD27抗体- Azide free (ab10456)

概述

  • 产品名称Anti-CD27抗体- Azide free
    参阅全部 CD27 一抗
  • 描述
    山羊多克隆抗体to CD27 - Azide free
  • 特异性This antibody does not cross react with recombinant human CD30, recombinant human NGF R, recombinant human soluble TNF RI, and recombinant human soluble TNF RII.
  • 经测试应用适用于: ELISA, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human CD27 extracellular domain expressed in Sf2I insect cells.

  • 阳性对照
  • 常规说明Prior to reconstitution, store at -20 °C. Reconstituted product may be stored at 2-8 °C for at least one month. For prolonged storage, freeze in working aliquots at -20°C.

性能

应用

Our Abpromise guarantee covers the use of ab10456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
Flow Cyt
WB
  • 应用说明ELISA: Use at a concentration of 0.5 - 1 µg/ml in indirect ELISA (determined to detect a limit of ~0.06 ng/well of human CD27).
    Flow Cyt: Use 0.5µg for 106 cells in indirect flow cytometry (determined using the appropriate secondary reagent for indirect immunofluorescence staining of T-lymphocytes).
    WB: Use at a concentration of 0.1 - 0.2 µg/ml (determined using human CD27 at 5ng/lane and 20ng/lane under non-reducing and reducing conditions, respectively). Predicted molecular weight: 31 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
    • 组织特异性Found in most T-lymphocytes.
    • 序列相似性Contains 3 TNFR-Cys repeats.
    • 翻译后修饰Phosphorylated and O-glycosylated.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 27 antibody
      • CD27 antibody
      • CD27 antigen antibody
      • CD27 molecule antibody
      • CD27_HUMAN antibody
      • CD27L receptor antibody
      • LPFS2 antibody
      • MGC20393 antibody
      • OTTHUMP00000238557 antibody
      • S152 antibody
      • T cell activation antigen CD27 antibody
      • T cell antivation antigen S152 antibody
      • T-cell activation antigen CD27 antibody
      • T14 antibody
      • TNFRSF 7 antibody
      • TNFRSF7 antibody
      • TNFSF7 antibody
      • Tp 55 antibody
      • Tp55 antibody
      • Tumor necrosis factor receptor superfamily member 7 antibody
      see all

    Anti-CD27 antibody - Azide free (ab10456)参考文献

    ab10456 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (Leukaemia cell lines)
    Loading amount 10 µg
    Specification Leukaemia cell lines
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in PBS-
    Username

    Dr. Mark Holland

    Verified customer

    提交于 Mar 30 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"